Abstract
Monitoring Bilirubin Binding Parameters in a Cohort of Japanese Neonates
Highlights
Besides its transport and osmotic pressure stabilizing functions, albumin (Alb) can bind unconjugated bilirubin to sequester the toxin within the vascular space and to buffer the level of unbound bilirubin (UB) that can cross membranes and enter cells
Previous studies using various direct assays have shown that the binding of bilirubin in newborns is lower than that expected from one-to-one binding of bilirubin to Alb as reported for adults and that this difference appears to increase as gestational age (GA) decreases [14,15,16]
Hmf using a prototype device (Bili- 4, Aviv Biomedical, Inc, Lakewood, NJ, USA) and UB by POD using colorimetry (Arrows-2 Analyzer). Such a comparison was made years ago using the original Aviv Hmf system and the original, non-automated POD assay [23], here we present data from a neonatal population spanning a full range of GAs, using the same blood samples collected for measurements of Alb, bilirubin binding capacity (BBC), bound to Alb (BB), and UB
Summary
Besides its transport and osmotic pressure stabilizing functions, albumin (Alb) can bind unconjugated bilirubin to sequester the toxin within the vascular space and to buffer the level of unbound bilirubin (UB) that can cross membranes and enter cells. A low Alb level that is reflective of a low bilirubin binding capacity (BBC), increases an infant’s risk of developing bilirubin-induced neurologic dysfunction (BIND) by reducing an infant’s ability to “tolerate” a given bilirubin load [1,2,3,4,5,6]. Previous studies using various direct assays have shown that the binding of bilirubin in newborns is lower than that expected from one-to-one binding of bilirubin to Alb as reported for adults and that this difference appears to increase as GA decreases [14,15,16]. Hmf determines the capacity of the single strong Alb site to bind bilirubin [15,17,18,19,20,21]. Within the limits of the singlesite binding model, the ratio of BB/RABC is proportional to the UB level with the proportionality constant being 1/Ka, where Ka is the affinity constant of the strong site [17,22,23]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
