Abstract
The genetic diversity among Tunisian pomegranate cultivars has been investigated. Using universal primers, the random amplified polymorphic DNA (RAPD) method was used to generate banding profiles from a set of twelve cultivars. Data was then computed with appropriate programs to construct a dendrogram illustrating the relationships between the studied cultivars. Our data proved the efficiency of the designed method to examine the DNA polymorphism in this crop since the tested primers are characterized by a collective resolving power of 12.83. In addition, the cluster analysis has exhibited a parsimonious tree branching independent from the geographic origin of the cultivars. In spite of the relatively low number of primers and cultivars, RAPD constitutes an appropriate procedure to assess the genetic diversity and to survey the phylogenetic relationships in this crop.
Highlights
The pomegranate (Punica granatum L.), a woody perennial shrub or small tree, is native of Persia [1] and is one of the oldest known edible fruit trees
Diversity in Punica granatum has been investigated by random amplified polymorphic DNA (RAPD) [8,9,10] and AFLP [11,12], and recently 18S-28S rDNA intergenic spacerRFLP has been used for pomegranate cultivar identification [13]
We report the usefulness of the RAPD method to assess the molecular genetic diversity of
Summary
The pomegranate (Punica granatum L.), a woody perennial shrub or small tree, is native of Persia [1] and is one of the oldest known edible fruit trees It has been cultivated extensively in the Mediterranean countries such as Tunisia, Turkey, Egypt, Spain, and in some extent in California, China, Japan and Russia [2]. Cytoplasmic genetic effects on morphology constitute an additional factor contributing to ambiguities encountered in the results of key-derived genotype classification This leads to a lack of information and a decrease in the success of clone differentiation based on morphological characteristics [7]. We report the usefulness of the RAPD method to assess the molecular genetic diversity of Tunisian pomegranate
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