Abstract

Objective To investigate the effect and mechanism of DHA on the cell proliferation, apoptosis, and migration in human liver cancer cell line Huh-7 with mutant TP53 in vitro. Methods MTT assay was used to detect the inhibitory effect. Immunofluorescence assay and WB were applied to detect the expression of γH2AX after treatment of DHA. FCM was employed to assess the apoptosis rate. Scratch method was used to test the cell migration ability. Western blot was used to determine the expression of p-STAT3(Y705). Results DHA inhibited the cell proliferation in Huh-7 cells.The results of immunofluorescence assay and WB showed that DHA enhanced the expression level of γH2AX and induced the DNA double-strand break (DSB) in Huh-7 cells. WB showed that DHA downregulated the phosphorylation of STAT3 at the Tyr705. Conclusions DHA caused the DSB and induced apoptosis in Huh-7 cells regardless of p53 status. DHA inhibited cell proliferation and migration of Huh-7 cells. It is probable that DHA induced apoptosis via downregulation of p-STAT3 (Tyr705). Key words: Hepatocellular carcinoma; Dihydroartemisinin; Cell proliferation; Cell apoptosis

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