Abstract
Leaf rust caused by Puccinia triticina Eriks. is the most prevalent wheat rust worldwide and occurs annually wherever wheat is grown. The most economical and environmentally friendly method to control this fungal disease is genetic resistance, which is achieved through deploying effective resistance genes. Tightly-linked molecular markers facilitate gene tagging and their deployment with other resistance genes, which in turn contribute to durable leaf rust resistance. The leaf rust resistance gene Lr36 derived from Aegilops speltoides Taush and introgressed into hexaploid wheat by backcrossing, is located on chromosome 6BS. Despite detection of low frequencies of virulence for this gene, no tightly-linked marker is available in disease resistance breeding. Therefore, this research aimed at analysis of simple sequence repeats (SSR) markers linked with Lr36 in 171 individuals of an F2 population from a cross between the Lr36-carrying line (ER84018) and the susceptible cultivar; Boolani. Of 36 primer pairs on chromosome 6BS tested for polymorphism in parents and resistant and susceptible bulks, eight were polymorphic of which the markers Xgwm88 and Xcfd13 flanked Lr36 by 3.8 and 5.2 cM, respectively. The identified markers were validated on 35 genotypes with different genetic backgrounds including few Australian wheat leaf rust differential sets, Iranian landraces and cultivars, and several cultivars and/or breeding lines from Switzerland and Afghanistan, which confirmed that they can be used in selection for Lr36 in wheat breeding programs.
Highlights
Wheat (Triticum aestivum) is a strategic crop all over the world
Based on Polymerase chain reactions (PCR) amplifications, the primer barc198 produced a band of 130 bp in ER84018 while it amplified a 150 bp band in Boolani
Though the Aegilops speltoides-derived leaf rust resistance gene, Lr36, has been mapped on the short arm of 6B (Dvorak and Knott 1990), it has not been deployed in wheat cultivars likely due to the unavailability of a diagnostic marker for its selection
Summary
Wheat (Triticum aestivum) is a strategic crop all over the world. Its production is often threatened by biotic and abiotic stresses (Arzani and Ashraf 2017). Fungal diseases including the three rusts; leaf, stripe, and stem rusts, result in significant annual yield losses and detrimental effects on seed quality (McIntosh 1998). Is the most prevalent wheat rust worldwide and occurs annually wherever wheat is grown (Kolmer 2013). The most economical and environmentally friendly method to control this disease is genetic resistance. Rust pathotypes can mutate to virulence on wheat genotypes with previously effective resistance genes. Breeding for rust resistance can be a never-ending task that has to be supported by ongoing pathogenicity surveys to confirm continued resistance gene effectiveness
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