Abstract
The optical microscope relies on spatial variations of refractive index, absorption and reflectivity in a specimen in order to produce the modulation in light intensity necessary to form the magnified image. The ability to identify the materials which comprise the specimen is very limited as these properties are generally not material specific. By contrast the Raman spectrum of a material is usually not only sufficient to give positive identification, but detail in the spectrum can often be used to assess the physical state of the material. Thus images recorded in light scattered in a particular Raman band should be very informative about the distribution of different molecular species.We have developed a Raman microscope which uses multilayer dielectric filters to select the Raman band with which the image is formed. The optical quality of the filters is sufficiently high so as not to degrade the image forming capabilities of the microscope. The filters typically have a pass band of 20 cm-1 and are wavelength tuned by rotation.
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