Abstract
Because there was no such study done on identification of tick species by PCR technique in in Duhok Governorate, therefore present study was done to identify tick species by using molecular study by using of 16S rRNA and DNA sequencing. About 1000 ticks were collected from both sheep and goat, form Duhok Governorate including: Barwaria, Zakho, Sumeil, Mangeshik, Sersing, Shekhan and Akre, between May and June 2016, between April and June 2017. The result found during this study were six species under two genera of the hard ticks were identified by molecular study and sequencing including: three species were under the genus Hyalomma and three species were under the genus Rhipiciphalus that infect small ruminants in Duhok governorate from these species a new species under the Hylomma genra (Hyalomma asiatium asiaticum) with accession number (MN594484), was first time reported in Duhok governorate. Also phylogenetic tree was constructed depend on the 16S rRNA.
Highlights
Tick is obligate ectoparasite that infests both animal and human and causing many diseases, due to they act as a vector of some pathogens including: protozoa, bacteria and virus [13]
The result found during this study were six species under two genera of the hard ticks were identified by molecular study and sequencing including: three species were under the genus Hyalomma and three species were under the genus Rhipiciphalus that infect small ruminants in Duhok governorate from these species a new species under the Hylomma genra (Hyalomma asiatium asiaticum) with accession number (MN594484), was first time reported in Duhok governorate
Six species under two genera of the hard ticks were identified by molecular study and sequencing including three species were under the genus Hyalomma and three species were under the genus Rhipiciphalus that infect small ruminants in Duhok governorate from these species a new species under the Hylomma genra (Hyalomma asiatium asiaticum) with accession number (MN594484), was recently reported in Duhok governorate, Iraq were identify as (Table 3)
Summary
Tick is obligate ectoparasite that infests both animal and human and causing many diseases, due to they act as a vector of some pathogens including: protozoa, bacteria and virus [13]. There are about 878 tick species; most of these are under the two famous families including: Ixodidae and Argasidae [4]. The first group, Prostriata that include only the Ixodid and is considered the largest genus and relatively have 217 known species and the other ticks are arranged under the second group Metastriata including Amblyomma, Anocentor, Apanomma, Haemaphysalis, Hyalomma, Rhipicephalus, Boophilus and Dermacentar [6]. During this study 16S rRNA gene was used for the identification of species of hard ticks that infested sheep and goats among Duhok governorate, Iraq. Because there was no such study done on identification of tick species by PCR technique in north of Iraq and in Duhok governorate, Iraq
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