Abstract
Mango (>Mangifera indica) belonging to Anacardiaceae family is a fruit that grows in tropical regions. It is considered as the King of fruits. The present work was taken up to identify a tool in identifying the mango species at the molecular level. The chloroplast trnL-F region was amplified from extracted total genomic DNA using the polymerase chain reaction (PCR) and sequenced. Sequence of the dominant DGGE band revealed that Mangifera indica in tested leaves was Mangifera indica (100% similarity to the ITS sequences of Mangifera indica). This sequence was deposited in NCBI with the accession no. GQ927757. AFLP - Amplified fragment length polymorphism , cpDNA - Chloroplast DNA, DDGE - Denaturing gradient gel electrophoresis, DNA - Deoxyribo nucleic acid, EDTA - Ethylenediamine tetraacetic acid, HCl - Hydrochloric acid, ISSR - Inter simple sequence repeats, ITS - Internal transcribed spacer, MATAB - Methyl Ammonium Bromide, Na(2)SO(3) - Sodium sulphite, NaCl - Sodium chloride, NCBI - National Centre for Biotechnology Information, PCR - Polymerase chain reaction, PEG - Polyethylene glycol, RAPD - Randomly amplified polymorphic DNA, trnL-F - Transfer RNA genes start codon- termination codon.
Highlights
Mango (Mangifera indica) is a fruit that grows in tropical regions belonging to Anacardiaceae Lindl., the cashew family which includes more than 700 species in 82 genera that are primarily distributed pantropically
The global spread of mango outside its original centres of domestication probably did not occur until the beginning of the European voyages of the 15th and 16th centuries, when the Portuguese took the mango to West-Africa and from there to Brazil at the beginning of the 18th century, and when the Spanish introduced polyembryonic mango types from The Philippines to Central America through the Pacific trading ports of Mexico and Panama
Collection of Plant material: Fresh leaves of Mango, Mangifera indica L.var.totupura were collected from Nuziveedu, Krishna District, Andhra Pradesh, India were used in this study for DNA extraction
Summary
Background: Mango (Mangifera indica) is a fruit that grows in tropical regions belonging to Anacardiaceae Lindl., the cashew family which includes more than 700 species in 82 genera that are primarily distributed pantropically. Materials and Methodology: Plant materials and DNA isolation: Fresh leaves of Mango, Mangifera indica L.var.totupura used in this study for DNA extraction were collected from Nuziveedu, Krishna District, Andhra Pradesh, India. DNA amplification and sequencing: The chloroplast trnL-F regions were amplified from extracted total genomic DNA using the polymerase chain reaction (PCR) method.
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