Molecular docking analysis of Ficus religiosa L. fruit extracts with HMG-COA reductase inhibitors, anti-hyperlipidemic effects of its in Albino wistar rats, triggered by Triton-X 100 and High fat diet

Abstract

Hyperlipidemia is the major risk factor for coronary artery disease in various patients suffering with cardiovascular disorders. Literature claims that flavonoids and phenolic compounds are able to reduce hyperlipidemia. Aim of the present study is to establish antihyperlipidemic activity of Ficus religiosa L. fruits extract with molecular docking approach and perform in-vivo study.Extraction has performed by Soxhlet apparatus with methanol for 72 h. Further, the extract has subjected to phytochemical tests followed by GC-MS analysis. Molecular docking study has been conducted to screening the compounds in extract which have affinity to HMG-COA reductase (PDB ID: 1HWK) receptor with reference to Atorvastatin. Anti-hyperlipidemic activity of the extract has investigated by studying in vivo effects on Triton-X 100 and High fat diet induced animal model. The animals were divided in 5 groups of 6 each. The extract has administered at a dose of 1000 mg/kg (p.o) and 500 mg/kg (p.o) to both models. Atorvastatin was used as standard.The GC-MS result of Ficus religiosa L. fruits has demonstrated the presence of phenols, flavonoids. Molecular docking study has been performed to identify the anti-oxidant compounds in the extracts. The extract has shown a significant decrease in the levels of Body weight, serum cholesterol, glucose, triglyceride, LDL, VLDL and significant increase in the level of serum HDL. The result of the research has showed that Ficus religiosa L. fruits possess antihyperlipidemic activity in both groups; the results were statistically significant compared to Atorvastatin.