Abstract
This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre). Skin biopsy imprints or biopsy fragments were assayed via kDNA-PCR/RFLP and FRET-real-time PCR. Of individuals with suspected CL, 18 were positive for Leishmania kDNA. Leishmania (Viannia) braziliensis (61.1%) and Leishmania (Viannia) guyanensis (5.5%) were identified in the positive samples. These results are congruent with the previous reports in Acre and Bolivia, revealing L. braziliensis as the most prevalent species. L. guyanensis identification also corroborates with the epidemiology of the disease in the Amazon Basin.
Highlights
Introduction: This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre)
Following HaeIII digestion of the kinetoplast DNA (kDNA) amplified products from 18 positive samples, the restriction profile corresponding to L. (V.) braziliensis was identified in 11/18 samples (61.1%), namely the presence of two fragments of 40 and 80bp in 12% silver-stained acrylamide gel
Infection with L. (V.) braziliensis in 61.1% of the patients suggests that this species is the main etiological agent responsible for CL cases in the municipality of Brasiléia, which agrees with previous findings in Rio Branco and other areas in Bolivia and Peru, bordering the State of Acre[6,7,8,13]
Summary
Abstract Introduction: This study proposes to identify the Leishmania species found in the skin lesions of cutaneous leishmaniasis (CL) patients from Brasiléia municipality (Acre). Results: Of individuals with suspected CL, 18 were positive for Leishmania kDNA. Twenty-two patients who provided clinical and sociodemographic information and presented positive results in the Montenegro skin test were included (Table 1).
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