Abstract
The aim of this study was to understand the role of TiERF1 and RC7 genes in wheat defense response to take-all.Both genes were simultaneously transformed into wheat cultivar Yangmai 18.The resistance to talk-all in five transgenic lines were analyzed by PCR and RT-PCR methods,and evaluated by artificial inoculation in greenhouse and field tests.Scanning electron microscope technique was used to observe the morphology and number of the pathogen mycelia on the root surfaces of resistant transgenic and susceptible receptor wheat plants.The PCR assay showed that both TiERF1 and RC7 genes were integrated into the transgenic lines and inherited to the T3generation.The RT-PCR assay confirmed expressions of both genes in the transgenic lines.The results showed that five transgenic lines exhibited stable and efficient resistance to take-all with disease severity lower than10% at seedling stage in greenhouse and rate of white spike lower than 13% at maturity in field.In the contrast of Yangmai 18,the severity was 62.98%,rate of white spike was 26.09%.Electron microscope observation revealed that the number and growth tendency of hyphae on roots of the resistant transgenic wheat were significantly lower and weaker than those of the wild type.The expressions of TiERF1 and RC7 inhibit the growth of pathogen hyphae in the transgenic wheat plants,leading to improved resistance to take-all.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
