Abstract
小麦纹枯病(主要病原为禾谷丝核菌)和赤霉病(主要病原为禾谷镰刀菌)已成为我国小麦生产的重要病害。 TaPIEP1 是从小麦中分离到的1个病原诱导的基因，其编码蛋白是可与GCC-box顺式元件结合、转录激活型的ERF转录因子。本研究以8个转 TaPIEP1 基因小麦株系的T 4 和T 5 代植株为试材，进行了外源转 TaPIEP1 基因的PCR检测、Southern杂交、RT-PCR与Q-RT-PCR的分析以及纹枯病菌、赤霉病菌接种与抗性鉴定。结果表明，外源 TaPIEP1 基因在转基因小麦中能够稳定遗传，以单拷贝或双拷贝整合到7个转基因小麦株系基因组的不同位点；外源 TaPIEP1 基因在转基因小麦中能超量表达；与受体扬麦12相比， TaPIEP1 表达水平高的8个转基因小麦株系对纹枯病抗性显著提高，4个株系中一些材料对赤霉病抗性显著提高，3个株系中一些材料兼抗纹枯病和赤霉病，说明 TaPIEP1 正向参与了小麦对纹枯病和赤霉病抗性反应，利用该基因通过基因工程可创制抗纹枯病、赤霉病的小麦新种质。
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