Abstract
BackgroundPseudomonas aeruginosa is a common bacterium that can cause disease in humans and other animals. This study was conducted to screen for molecular detection and antimicrobial-resistant P. aeruginosa in Musca domestica in different locations in the Iranian provinces of Shahrekord and Isfahan.MethodsMusca domestica were captured by both manual and sticky trap methods, during the daytime, from household kitchens, cattle farms, animal hospitals, human hospitals, slaughterhouses and chicken farms at random locations in Shahrekord and Isfahan provinces of Iran, and subsequently transported to the laboratory for detection of P. aeruginosa. In the laboratory, flies were identified and killed by refrigeration in a cold chamber at −20 °C, then placed in 5 mL peptone water and left at room temperature for five hours before being processed. Pseudomonas isolates were preliminarily identified to genus level based on colony morphology and gram staining, and their identity was further confirmed by polymerase chain reaction.ResultsOverall blaTEM gene was recovered from 8.8 % (53/600) of the P. aeruginosa isolated from houseflies collected from the two provinces. A slightly higher prevalence (10.7 %; 32/300) was recorded in Shahrekord province than Isfahan province (7.0 %; 21/300). The locations did not differ statistically (p < 0.05) in bacterial prevalence in flies. Seasonal prevalence showed a significantly lower infection frequency during autumn.ConclusionsHouseflies are important in the epidemiology of P. aeruginosa infections.
Highlights
Pseudomonas aeruginosa is a common bacterium that can cause disease in humans and other animals
The overall prevalence of P. aeruginosa in houseflies in this study was 8.8 % (53/600) from houseflies collected from the two provinces
The 10.7 % (32/300) prevalence of flies obtained in Shahrekord province was slightly higher than the 7.0 % (21/300) found in Isfahan province
Summary
Pseudomonas aeruginosa is a common bacterium that can cause disease in humans and other animals. This study was conducted to screen for molecular detection and antimicrobial-resistant P. aeruginosa in Musca domestica in different locations in the Iranian provinces of Shahrekord and Isfahan. Houseflies (Musca domestica) are the most common of domestic flies Their feeding and reproductive habits make them important mechanical and biological vectors of several human and veterinary pathogens including those causing nosocomial, enteric and anthropozoonotic infections [1,2,3,4,5]. This study was conducted to screen for Hemmatinezhad et al Journal of Venomous Animals and Toxins including Tropical Diseases (2015) 21:18 molecular detection and antimicrobial-resistant P. aeruginosa in Musca domestica in different locations in the Iranian provinces of Shahrekord and Isfahan
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