Abstract
Molecular cloning, tissue distribution and ontogenetic regulation of sodium/proton exchanger isoform 2 (NHE2) mRNA expression were evaluated in the pig small intestine during postnatal development. The 2872‐bp porcine full cDNA sequence of the NHE2 (EF672046) cloned in this study showed 80% and 70% homology with known human and mouse gene sequence, respectively. Hydrophobic prediction suggests 13 putative membrane‐spanning domains within porcine NHE2. The porcine NHE2 mRNA was detected in brain, liver, kidney, heart, lung, small intestine and muscle. The small intestine had the highest NHE2 mRNA abundance and the brain, lung and liver had the lowest NHE2 mRNA abundance (P < 0.05). Along the longitudinal axis, the duodenum had the highest NHE2 mRNA abundance and the ileum and colon had the lowest NHE2 mRNA abundance (P < 0.05). The NHE2 mRNA level was increased from the day 1 to day 26 in the duodenum (P < 0.05) and dropped dramatically on day 30 (P < 0.05). There is no difference between day 1 and day 7 (P > 0.05). After day 30, the NHE2 mRNA level stayed at the same except on day 90 (P > 0.05). The mRNA expression of NHE2 was not only differentially regulated by age but also differentially distributed along the small intestine of piglets at early stages and growing stages of life, which may contribute to changes in NHE activity.
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