Abstract
The pituitary growth hormone (GH) gene of silver sea bream ( Sparus sarba) was cloned and characterized and found to be 615 base pairs encoding a protein of 204 amino acids. Using a bacterial expression system, recombinant protein was prepared and rabbit polyclonal antibody was raised. Transcript and protein amounts of GH were measured in fish that were adapted to a range of salinities, acclimated to different temperatures, or undergoing a natural time course of Vibrio alginolyticus infection. Isoosmotic salinity (12 ppt) adaptation resulted in increased GH transcript and protein in comparison to freshwater (0 ppt) and seawater (33 ppt) adapted fish. It was also found that cold temperature (12 °C) acclimated sea bream had higher amounts of pituitary GH transcript and protein when compared to warm temperature (25 °C) acclimated fish. Finally, the amounts of GH transcript and protein were found to be rapidly downregulated from an early stage of disease. The results from the present study demonstrate how GH can be modulated during both abiotic and biotic stress in fish.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
