Abstract
Growth hormone (GH) is a polypeptide produced in the anterior pituitary lobe that triggers different biochemical pathways and increases cell proliferation and growth. In this work, the pNic based vector for periplasmatic secretion was developed. Recombinant human growth hormone was produced via periplasmatic secretion (42h, 30 °С) followed by several steps of purification. GH obtained does not contain His-tag. Expression level of 2,85 mg per1 literof bacterial culture was achieved.
Highlights
Growth hormone (GH) is a polypeptide produced in the anterior pituitary lobe that triggers different bioche mical pathways and increases cell proliferation and growth
Recombinant human growth hormone was produced via periplasmatic secretion (42h, 30 °С) followed by several steps of purification
High-level expression and purification of recombinant human growth hormone produced in soluble form in Escherichia coli / Z
Summary
Growth hormone (GH) is a polypeptide produced in the anterior pituitary lobe that triggers different bioche mical pathways and increases cell proliferation and growth. Конструирование экспрессионного вектора для периплазматической экспрессии соматотропина человека в клетках E. coli. При создании конструкции для периплазматической экспрессии соматотропина человека использовался вектор pNic, содержащий лидерный пептид pelB, 10 His- Tag, сайт узнавания TEV-протеазы, сайт для LIC-клонирования и ген устойчивости к канамицину.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings of the National Academy of Sciences of Belarus, Chemical Series
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
