Abstract
The present study aimed to investigate changes in the protein contents using SDS-PAGE, also using the random amplified polymorphic DNA (RAPD-PCR) assay to identify changes in DNA concentration in the embryos of Egyptian toad Bufo regularis. Our experiment started when tadpoles began to feed. The adapted embryos were divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Cluster method was used to indicate the distinct distance between the band patterns of different stages. An increase occurred in the concentration of protein fractions in these tadpoles upon metamorphosis and that when metamorphosis was completed. DNA concentration exhibited an evident low values at the premetamorphic stage 44 (105.85 ± 0.519 ng/μl) compared with the other more advanced developmental stages 55 and 56 (204.056 ± 0.651 ng/μl and 234.55 ± 2.325 ng/μl respectively). In conclusion, acquire information about the changes in the molecular content of Bufo regularis during metamorphosis has been estimated.
Highlights
Bufonidae represent one of the most anuran families distributed in all parts of the world except for Antarctica [1]
Changes in the concentration of the total protein in different developmental stages of B. regularis were shown in Table 2, total protein concentration showed slightly increased in different developmental stages, except in stage 48 and 55 (Third indentation of the foot paddle) slightly decreased in total proteins concentration was recorded
These similar to [26] where they observed that an increase in the number of blood serum proteins of P. bedriagae especially in the concentrations of albumin and globulin fractions. [49] stated that an increase occurred in the concentration of serum protein fractions in anurans upon metamorphosis and that when metamorphosis was completed, while [50] has reported that the embryo proteins of Bombina variegate [51], and Ichthyosaura alpestris increased in stages 28 to 36 [52]
Summary
Bufonidae represent one of the most anuran families distributed in all parts of the world except for Antarctica [1]. About 300 species was included in the Bufo genus [1]. The common species in Egypt were Bufo regularis, Bufo viridis, Rana bedriagae and Ptychadena mascareniesis [1]. Due to their habitation, Bufonidae was fre-. (2014) Molecular Characterization on the Early Developmental Stages of the Egyptian Toad Bufo regularis Reuss. The few studies refer to the molecular characterization of this species [2] [3]
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