Abstract
Copper/zinc superoxide dismutases (Cu/ZnSODs) play important roles in improving banana resistance to adverse conditions, but their activities depend on the copper chaperone for superoxide dismutase (CCS) delivering copper to them. However, little is known about CCS in monocots and under stress conditions. Here, a novel CCS gene (MaCCS) was obtained from a banana using reverse transcription PCR and rapid-amplification of cDNA ends (RACE) PCR. Sequence analyses showed that MaCCS has typical CCS domains and a conserved gene structure like other plant CCSs. Alternative transcription start sites (ATSSs) and alternative polyadenylation contribute to the mRNA diversity of MaCCS. ATSSs in MaCCS resulted in one open reading frame containing two in-frame start codons to form two protein versions, which is supported by the MaCCS subcellular localization of in both cytosol and chloroplasts. Furthermore, MaCCS promoter was found to contain many cis-elements associated with abiotic and hormonal responses. Quantitative real-time PCR analysis showed that MaCCS was expressed in all tested tissues (leaves, pseudostems and roots). In addition, MaCCS expression was significantly induced by light, heat, drought, abscisic acid and indole-3-acetic acid, but inhibited by relatively high concentrations of CuSO4 and under cold treatment, which suggests that MaCCS is involved in abiotic and hormonal responses.
Highlights
Bananas are one of the most important crops in tropical and subtropical regions
The copper chaperone for Cu/ZnSOD (CCS) functions as an intracellular copper shuttle to deliver the copper to apo-Cu/ZnSOD proteins to activate them [6,7]
A BLASTp search in the NCBI database showed that it is highly similar to the CCSs from Elaeis guineensis (72.2% identity), Zea mays (60.0% identity), Vitis vinifera (62.6% identity) and Arabidopsis thaliana (54.7% identity), which suggests that it belongs to the plant CCS gene family
Summary
Bananas are one of the most important crops in tropical and subtropical regions. They are susceptible to various environmental stresses, such as cold and drought, during their growth and development. Stress conditions often lead to the excessive accumulation of reactive oxygen species in cells, which results in metabolic malfunctions and cell death [1]. To reduce the damage caused by reactive oxygen species, plants employ efficient and complex antioxidative response systems, including superoxide dismutases [2]. The zinc required for the structural stability of the Cu/ZnSOD protein can be obtained by passive diffusion, while the copper in Cu/ZnSOD essential for the disproportionation of superoxide can only be acquired through a copper chaperone under normal physiological conditions [4,5]. The copper chaperone for Cu/ZnSOD (CCS) functions as an intracellular copper shuttle to deliver the copper to apo-Cu/ZnSOD proteins to activate them [6,7]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
