Abstract
Lactobacillus reuteriG4 contains a 7.0-kb plasmid (pTC82) encoding resistance to chloramphenicol (Cmr). Determination of the nucleotide sequence of the genetic determinant (cat-TC) encoding resistance to Cm on pTC82 revealed an open reading frame for a 238-amino-acid Cm acetyltransferase (CAT) monomer. This structuralcatgene, 714 bp in length, was highly related (ca. 95% nucleotide and ca. 81% amino acid identity) to the 648-bpcatgene fromStaphylococcus aureusplasmid pC194. A total of 6 bp transversions and 4 bp deletions was observed along the whole DNA sequence ofcat-TC compared to that ofcat-pC194. To determine the activity of the putativecat-TC gene, recombinant plasmid pUC8217 containing thecatdeterminant from pTC82 was subjected to a maxicell analysis. The observed molecular mass of the synthesized protein, based on electrophoretic mobility, was in reasonable agreement with the 27.3 kDa predicted from the DNA sequence. This is the first reported nucleotide sequence of a Cm-resistance determinant fromL. reuteriand also the first evidence of addingLactobacillusto the list of versatile bacterial genera which naturally acquire thecat-pC194 gene in the microbial ecological system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
