Abstract
Cathepsin L genes, which belonged to cysteine proteases, were a series of multifunctional protease and played important roles in a lot of pathological and physiological processes. In this study, we analyzed the characteristics a cathepsin L (named Mn-CL2) in the female oriental river prawn, Macrobrachium nipponense which was involved in ovary maturation. The Mn-CL2 was1,582 bp in length, including a 978 bp open reading frame that encoded 326 amino acids. The Mn-CL2 was classified into the cathepsin L group by phylogenetic analysis. Real-time PCR (qPCR) analysis indicated that Mn-CL2 was highly expressed in the hepatopancreas and ovaries of female prawns. During the different ovarian stages, Mn-CL2 expression in the hepatopancreas and ovaries peaked before ovarian maturation. In situ hybridization studies revealed that Mn-CL2 was localized in the oocyte of the ovary. Injection of Mn-CL2 dsRNA significantly reduced the expression of vitellogenin. Changes in the gonad somatic index also confirmed the inhibitory effects of Mn-CL2 dsRNA on ovary maturation. These results suggest that Mn-CL2 has a key role in promoting ovary maturation.
Highlights
Macrobrachium nipponense, called oriental river prawn, is an important freshwater commercial prawn in China
The full-length cathepsin L2 cDNA sequence of M. nipponense was obtained from the transcriptomes of ovary
All sequences of the ovary transcriptome data were deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive under Bioproject PRJNA541783
Summary
Macrobrachium nipponense, called oriental river prawn, is an important freshwater commercial prawn in China. Female prawns have the characteristics of “Short period of sexual maturity” [1]. After entering into the breeding season, females can reach to sexual maturity after being raised for about 45 days, and can reproduce multiple generations in the same year. Especially the water temperature reached above 28°C, it takes only 15 days for females to complete an ovarian maturation cycle (from ovarian emptying to the spawning). It makes an ideal materials for studying crustacean reproduction, it has been an important problem for the prawn industry for many years. It is very important to find out the key genes of ovary maturation
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