Abstract
Pufferfish are considered a culinary delicacy but require careful preparation to avoid ingestion of the highly toxic tetrodotoxin (TTX), which accumulates in certain tissues. In this study, the tissue distribution of peroxiredoxin-1 from Takifugu bimaculatus was investigated. The peroxiredoxin-1 protein was obtained by in vitro recombinant expression and purification. The recombinant protein had a strong ability to scavenge hydroxyl radicals, protect superhelical DNA plasmids from oxidative damage, and protect L929 cells from H2O2 toxicity through in vitro antioxidant activity. In addition, we verified its ability to bind to tetrodotoxin using surface plasmon resonance techniques. Further, recombinant proteins were found to facilitate the entry of tetrodotoxin into cells. Through these analyses, we identified, for the first time, peroxiredoxin-1 protein from Takifugu bimaculatus as a potential novel tetrodotoxin-binding protein. Our findings provide a basis for further exploration of the application of peroxiredoxin-1 protein and the molecular mechanisms of tetrodotoxin enrichment in pufferfish.
Highlights
Takifugu bimaculatus is a carnivorous groundfish that belongs to the Tetraodontiformes and is widely distributed from the South Yellow Sea to the South China Sea
A quantitative polymerase chain reaction method was used to investiga3toeft1h6e distribution of prx-1 transcripts among the tissues of immature and mature T. bimaculatus (Figure 1)
When glutathione (G6 oSfH16) was used as a positive control, the GSH concentration associated with a 50% hydroxyl free radical clearance rate was 0.95 mg/mL. These findings showed that the rTbprx-1 protein possessed a strong hydroxyl free radical scavenging ability. manner
Summary
Takifugu bimaculatus is a carnivorous groundfish that belongs to the Tetraodontiformes and is widely distributed from the South Yellow Sea to the South China Sea. Its delicious, chewy taste and high economic value make this fish popular in Southeast Asian markets [1,2]. T. bimaculatus can survive at lower latitudes and can adapt to higher temperatures and salinities as compared to the other three cultural pufferfish species in China; farming production of T. bimaculatus has attracted much interest in recent years [3]. Pufferfish contain highly toxic tetrodotoxin (TTX), which poses a significant risk to food safety. Levels of TTX are significantly correlated with species, tissues, and seasons. The tissue distribution of TTX in pufferfish is highly variable between species and the toxicity is correlated with the aquatic environment [7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
