Molecular and taxonomical study of the genus Chara (Linnaeus 1753) in Israel

We report a new locality for Rueppel’s Snake-eyed skink (Ablepharus rueppellii) in Southern Israel – near Shivta Junction. This record extends the known distribution of this species in Israel by ~25km. We examined all known localities of this species in Israel and the adjacent Sinai Peninsula (Egypt), and discuss some discrepancies between them and currently published range maps, including the one produced by the IUCN.

The charophytes in many regions of the world are still poorly understood. This hampers the synthesis of distributional and ecological datasets at worldwide and continental scales, as well as complicates the generalization of species concepts for widely distributed and local taxa. To fill in the blanks for charophytes in the Caucasus and to improve our knowledge of species distribution areas in Eurasia, a field survey and study of available specimens from Dagestan (North Caucasus, Russia) was conducted based on morphological observation using light and scanning electron microscopy and molecular genetic analyses allowing for the precise identification and testing of the presence of cryptic and undescribed taxa. Nineteen new localities for seven Chara species and one Tolypella species, seven new species, and one new genus were identified in the studied region, and one new species in the Caspian Sea region was found. Some species records changed the outline or filled in the gaps in species distribution data. The presence of species distributed mainly in central Eurasia (C. globata, C. neglecta) with mainly Mediterranean–Middle Eastern species (C. gymnophylla) is notable for this region, as well as for other studied regions of the Caucasus characterized by a mixture combination of species with different distribution patterns. Chara gymnophylla was frequent in Dagestan, similar to the Mediterranean and Middle Eastern regions. Small brackish waterbodies on the coast of the Caspian Sea, freshwater mountain rivers, small associated waterbodies, and water reservoirs are the main habitats of charophytes in the studied region. Based on habitat preference and distribution in the Caucasus, recommendations for the protection of some species were suggested. The lack of endemic species among charophytes from Dagestan and Caucasus contrasts with the flora of terrestrial magnoliophytes that is rich in species endemism.

Israel has a long and rich history of herpetological research, with many studies of local reptiles and amphibians. Despite being one of the most thoroughly surveyed countries in the region, surprising discoveries about its herpetofauna continue to emerge. The gecko Hemidactylus turcicus is currently regarded as the only species of its genus in Israel. Based on genetic data and phylogenetic analyses, we confirm the occurrence of another Hemidactylus species in Israel. Specimens from the southern Arava Valley, until now thought to represent H. turcicus were found to belong to H. granosus, a species that until now was only known from Egypt, southern Jordan and Saudi Arabia. This research thus increases the number of Hemidactylus species in Israel to two and reveals a geographic connection between H. granosus populations in Egypt and the Sinai Peninsula, eastward through southern Israel into southern Jordan and southwards towards western and central Saudi Arabia.

Comparative analysis of algal communities in the rivers of northern and southern Israel as bearing on ecological consequences of climate change

Migrating Blackcaps Sylvia atricapilla were mist netted at the desert edge in northern Israel and in Elat (southern Israel) during spring and autumn migrations between 1970 and 1991. Birds in spring in northern Israel were representative of birds that had completed the crossing of the Sahara, while those in Elat still had to cross the 150 km of the Negev Desert, which separates Elat and northern Israel. In autumn, birds captured in northern Israel were representative of those about to cross the Sahara Desert, while those in Elat had already started to cross the desert. The data allowed analysis of seasonal and location differences in the physiological state of Blackcaps before and after crossing the Sahara. Data analysed included body mass, visible fat score and calculated fat content. Autumn migrants were in better physiological condition than spring migrants at both locations, probably as a consequence of their migration route through fertile areas in autumn compared with the crossing of the Sahara in spring. Body mass was less variable after the Sahara crossing in spring than before the crossing in autumn. In spring, 71% and 67% of the birds were fat depleted (fat scores 0 and 1) at Elat and in northern Israel, respectively, while in autumn 34% and 42% were fat depleted. Blackcaps at Elat were 1.6 g lighter than those in northern Israel in autumn and 1.9 g lighter in spring. Potential flight ranges were estimated on the basis of meteorological conditions and flight altitude of passerines above the Negev in Israel (northern Sahara edge) during migration and on a simulation model that considered both energy and water as potential limiting factors for flight duration and distance. The simulation model predicted that half of the Blackcaps that stopped over in Elat and the majority of those that stopped over in northern Israel could not make a nonstop flight over the Sahara Desert in autumn without the assistance of at least an 8 m per s tailwind. Such a wind would still not be sufficient for 34% of the birds in Elat and 42% in northern Israel, and clearly they had insufficient fat reserves to cross the Sahara in a single flight. Although the fattest Blackcaps had accumulated sufficient fat to enable them to traverse the Sahara in a single flight, they probably faced dehydration by at least 12% of their initial body mass when they reached the southern Sahara edge. These birds should use intermittent migration with stopovers at sites with drinking and feeding potential. Their decision to stop over during the day in the desert at sites with shade but without food and water would be beneficial if the meteorological conditions during daytime migration imposed greater risks of dehydration than at night. Spring migrants could not reach their breeding areas in Europe without feeding, but those examined in Elat could cross the remainder of the desert in a single flight.

Evolutionary divergence in Chenopodium and validation of SNPs in chloroplast rbcL and matk genes by allele-specific PCR for development of Chenopodium quinoa-specific markers

Floods rarely occur in hyper-arid deserts and little is known about the magnitude and frequency of sediment delivery from their basins, despite their importance to changes to the landscape, infrastructures and engineering activities. Sediment yield from the Nahal Nehushtan watershed (11.9 km2) located in the Timna Valley in southern Israel was determined by assessing stratigraphic sections in its 60-year reservoir deposits. Stratigraphic correlation between event couplets allowed for quantification of sediment yields representing 13 former flow and flood events. Based on the sediment volume in the reservoir, the 29.8 t km−2 year−1 average specific sediment yield is one of the lowest among other studied warm deserts. Among the event layers, the thickest layer, deposited by a flash flood caused by a single short rain event, contributed 31% of the total sediment yield. Based on event reservoir sedimentation from watersheds located in several hyper-arid areas in the Middle East and North America, we demonstrate that sediment yield increases with drainage area as expected and mean annual sediment yield increases in hyper-arid areas with flood frequency. Our quantitative results, together with previous studies of hyper-arid areas, provide complementary evidence of fluvial sediment transport—the main landscape designer in hyper-arid fluvial landscapes.

Population size, structure and phenology of an endangered salamander at temporary and permanent breeding sites

The chloroplast gene rbcL was sequenced to elucidate the evolution of the gymnosperm plant order Cycadales. In accordance with traditional systematics, the order Cycadales and the corresponding genera cluster as monophyletic clades. Among them, the genus Cycas forms a basal group. The genetic distances within the genus Encephalartos and between the sister groups Encephalartos, Lepidozamia and Macrozamia, are unexpectedly small, suggesting that the extant species are the result of Miocene and Pliocene speciation. Their distribution in Africa or Australia, respectively, may therefore rather be due to long-distance dispersal than to Cretaceous continental drift, as had previously been assumed. The rbcL sequences also indicate that the colonisation of Madagascar by Cycas thouarsii occurred only recently as the sequences of C. thouarsii and Cycas rumphii from Indonesia are identical. In contrast, the divergence of the Cycadaceae and Zamiaceae apparently occurred in the Mesozoic.

Brief video intervention to increase treatment-seeking among individuals living in a conflict zone: A randomized controlled trial

We examined 3747 teeth from 134 hares (Lepussp.) collected at 46 sites in Israel to test whether variation in epigenetic occlusal characters was linked to ecogenetic or phylogenetic factors. Collection sites encompassed a wide range of ecogeographical and climatic regimes. We compared data from Israeli hares with occlusal characters of 160 cape haresL. capensisfrom East Africa and 68 brown haresL. europaeusfrom central Europe. Only three teeth (I1, I2, M3) did not show occlusal variation. Thirty‐eight occlusal characters were derived from dental variants by dichotomous (0/1) scoring. Absence of association of character states among characters of single teeth indicated a lack of morphotypes. Epigenetic differentiation among hares from northern, central and southern Israel, and the two East African, and two central European samples, was revealed by pairwise C. A. B. Smith's ‘mean measures of divergence’ (MMD), based on frequencies of character states. Cluster analyses of MMD values revealed little epigenetic differentiation between northern and southern Israeli hares, but greater differentiation between central European and East African hares. Concordance of the MMD matrix with linear geographical distances among sampling regions was demonstrated by a Mantel test. No frequencies of character states exhibited significant changes across the climatic parameters among hares from northern and southern Israel. But the individual folding index, which expresses the degree of enamel on the occlusal surface, slightly decreased from north to south. Our results support a phylogenetic interpretation of occlusal character variation. Despite distinct differences in external appearance, hares from northern and southern Israel probably comprise a single species that encompasses two closely related geographical populations with a probable area of overlap. Israeli hares are intermediate between European brown hares and East African cape hares, with a slightly closer relationship to the cape hares.

Genetic diversity of Chara populations found in Israeli Negev Desert streams was analyzed using amplified fragment length polymorphism (AFLP) in 31 individuals. The adjacent streams Avdat and Aqev were used as study sites in the Ein Avdat National Park. A Jaccard phylogenetic tree, based on 468 loci, showed clear separation between four Chara species: C. vulgaris, C. contraria, C. gymnophylla and Chara sp. The last two species served as out groups from the Northern Israel. In both C. vulgaris and C. contraria, genetic differences were found between the populations originating in the two streams: higher private loci number (80 vs. 24 in C. vulgaris and 58 vs. 25 in C. contraria) and polymorphic loci level (45% vs. 23% in C. vulgaris and 39% vs. 21% in C. contraria) were found in Avdat compared to Aqev. The genetic divergence revealed within and between the two streams is presumably adaptive and determined by natural selection associated with ecological stress. Sunlight intensity, water level and pH were found to be the main ecological variables associated with species clustering, through selection, in the varying ecology of the sampling stations along each of the streams. Evidence for gene flow between and within the streams was found using the structure analysis, suggesting that sampling sites condition is the regulating factor for oospores establishing, and hence, gene flow occurs more often between sampling sites with similar ecological conditions.

Natural self-cleaning of oil-polluted beaches by waves

<p>Floods rarely occur in hyper-arid deserts and little is known about the magnitude and frequency of sediment delivery from their basins, despite their importance to changes to the landscape as well as to infrastructures and engineering activities. Sediment yield from the Nahal Nehushtan watershed (15.7 km<sup>2</sup>) located in the Timna Valley in southern Israel, was determined by assessing stratigraphic sections in its 60-year reservoir deposits. Stratigraphic correlation between event couplets allowed quantification of sediment yields representing 13 former flash-flood events. Based on the sediment volume in the reservoir, the 24.6 t km<sup>-2</sup> y<sup>-1</sup> average sediment yield is the lowest among other studied warm deserts. Among the event layers, five are voluminously small and seven are medium-sized. The thickest layer, deposited by a flash flood caused by a single short rain event, contributed 29% of the total sediment yield. This demonstrates the overarching effect of medium magnitude events on the rate of sediment production in a hyper-arid setting. Based on event reservoir sedimentation from watersheds located in several hyper-arid areas in the Middle East and North America, sediment load increases with drainage area as expected; however, sediment yield does not decrease with drainage area, as was shown for arid environments. Overall, mean annual sediment yield is very low and increases with flood frequency, with considerable variation generated by local characteristics. Our quantitative results together with previous studies of hyper-arid areas, provide complementary evidence of fluvial sediment transport - the main landscape designer in fluvial desert landscapes.</p>

Altogether, 4,008 sand flies belonging to seven species were collected over a period of one year in the micro-habitats of a single canyon in the Carmel Mountain ridge. The three most abundant were P. arabicus, P. tobbi, and P. simici. Our results suggest that none of the seven sand fly species was indifferent to the heterogeneity of the microenvironment inside the canyon. Apart from the rare P. perfiliewi, which was only collected on the upper part of the south-facing slope, and P. tobbi, which clustered on the north-facing slope, the bulk of the other sand flies were caught on the bottom of the canyon. During the summer, the catches of all sand fly species increased to reach their maximum number in August and September. In April and May, there was lush vegetation and humidity, so species were distributed evenly throughout their habitats. With the onset of summer dryness, the sand flies concentrated in the humid habitats. The rate of concentration was essentially higher for males than for females, and this variation may result from differences in the behavior of the two sexes. During our study, none of the 2,318 dissected female sand flies were positive for Leishmania promastigotes.