Abstract
The newly identified tomato necrotic stunt virus (ToNStV), with a genome of 10,057 nucleotides, had 60 % nucleotide sequence and 70 % coat protein amino acid sequence identity to other potyviruses. Analysis of a second ToNStV isolate revealed two single nucleotide substitutions. ToNStV induced stunting and chlorotic to necrotic leaf symptoms on tomato plants. In host-range assays, ToNStV systemically infected many species in the family Solanaceae and locally infected some species in the families Amaranthaceae, Brassicaceae and Cucurbitaceae. A sensitive and reliable real-time RT-PCR assay was developed using simply prepared crude tissue extract.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
