Abstract
Postovulatory ageing compromises oocyte quality and subsequent development in various manners. We aimed to assay the protective effects of mogroside V on porcine oocyte quality during in vitro ageing and explore the related causes. We observed that mogroside V can effectively maintain normal oocyte morphology and early embryo development competence after prolonged culture for 24 h. Moreover, mogroside V can markedly reduce reactive oxygen species (ROS) levels, alleviate spindle formation and chromosome alignment abnormalities, improve mitochondrial contents, adenosine triphosphate (ATP) levels and the membrane potential (ΔΨm), and reduce early apoptosis in aged oocytes. We examined the molecular changes and found that SIRT1 expression was decreased in in vitro aged oocytes but was maintained by exposure to mogroside V. However, when SIRT1 was successfully inhibited by the specific inhibitor EX-527, mogroside V could not reduce ROS levels or alleviate abnormal spindle organization and chromosome misalignment. In summary, our results demonstrated that mogroside V can alleviate the deterioration of oocyte quality during in vitro ageing, possibly by reducing oxidative stress through SIRT1 upregulation.
Highlights
Postovulatory oocyte ageing is a process in which MII oocytes undergo a time-dependent deterioration in quality when no fertilization takes place within the ideal time frame [1]
These results showed that mogroside V (MV) reduced the activation rate of the aged oocytes from 32.1 to 18.5% at 50 μM and 16.9% at 100 μM (Figure 1B)
We examined the effects of MV on attenuating porcine oocyte deterioration resulting from in vitro ageing and attempted to elucidate the related causes
Summary
Postovulatory oocyte ageing is a process in which MII (metaphase II) oocytes undergo a time-dependent deterioration in quality when no fertilization takes place within the ideal time frame [1]. Postovulatory ageing is harmful to preimplantation and mid-term gestational development, as well as the fitness of offspring [4] These defects may be caused by oxidative stress induced by postovulatory ageing [4]. Siraitia grosvenorii (Luo-han-guo, LHG) is an edible and medicinal plant primarily distributed in Guangxi, China [7]. Because of their sweetness (approximately 300-times sweeter than sucrose), low caloric value and low toxicity [8, 9], extracts made from the fruit of LHG are generally recognized as safe by the Food and Drug Administration (FDA) [4] and are used as an alternative to sucrose in many countries, such as Japan, America, Australia and New Zealand [10]. 46 triterpenoid compounds, including mogrosides I, III, IV and V, have been isolated from LHG [10], and mogroside V (MV) is the most abundant form and is the most well studied [7, 11, 12]
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