Abstract
The effect of Fasciola hepatica excretory–secretory products (FhESPs) on mitogen-induced proliferation of sheep peripheral blood mononuclear cells (PBMCs) and PBMC subsets (CD2 +, CD4 +, CD8 +, γδTCR + or CD21 + cells) were studied. PBMCs were incubated with Concanavalin A (ConA) or phytohemagglutinin (PHA) at optimal (1 μg per well) or suboptimal (0.25 μg per well) doses and with FhESPs at several doses (1.25–20 μg per well). PBMC subsets were incubated with ConA at a suboptimal dose and with FhESPs at 5 μg per well. These cells were incubated with or without monocytes (CD14 + cell). FhESPs slightly increased the proliferation of PBMCs stimulated with optimal doses of PHA. FhESPs (10 and 20 μg per well) inhibited the PBMCs stimulated with optimal doses of ConA. FhESP dose-dependent inhibition was observed on PBMCs stimulated with suboptimal doses of ConA. CD21 + lymphocytes (B lymphocytes), CD14 + cells (monocytes) and γδTCR + cells were not stimulated by ConA. T lymphocyte subsets (CD2 +, CD4 + or CD8 + cells) proliferation was decreased by FhESPs at 5 μg per well. FhESPs inhibits the ConA-induced stimulation of sheep PBMCs and sheep T lymphocyte subsets. Further studies should be done to investigate the mechanism of this FhESP immunomodulatory effect.
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