Abstract
Obesity is associated increased serum interleukin-6 (IL-6) levels. Adipose tissue has been shown to secret up to 35% of serum IL-6. However, only 10% of adipose tissue-derived serum IL-6 levels are accounted by the fully differentiated adipocytes. Thus, we previously tested and showed that adipose tissue stromal vascular cells and preadipocytes contribute significantly toward obesity-induced increases in plasma IL-6 levels. In our current study, we tested anti-inflammatory effects of n-3 polyunsaturated fatty acids (n-3 PUFA) including eicosapentaenoic acid (EPA, 20:5) and EPA precursor stearidonic acid (SDA, 18:4) on 3T3-L1 preadiopcytes and 3T3-L1 adipocytes. Results showed that EPA (120 μM, 86% decrease) and SDA (120 μM, 82% decrease) significantly reduced lipopolysaccharide (LPS, 200 ng/ml)-induced IL-6 secretion in 3T3-L1 preadiopcytes. Results also showed that EPA (120 μM, 24% decrease) and SDA (120 μM, 39% decrease) significantly reduced LPS-induced IL-6 secretion in 3T3-L1 adipocytes. Further, we showed that n-3 PUFA significantly reduced adipose tissue mesenchymal stem cell IL-6 secretion in a dose dependent manner. These data suggest that n-3 PUFAs have potent anti-inflammatory effects on adipose tissue cell derived IL-6 secretion.
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