Abstract
BackgroundThe Urabe AM9 vaccine strain of mumps virus contains two variants of V protein: VWT (of HN-A1081 viral population) and VGly (of HN-G1081). The V protein is a promoting factor of viral replication by blocking the IFN antiviral pathway.FindingsWe studied the relationship between V protein variants and IFN-α2b-induced apoptosis. V proteins decrease activation of the extrinsic IFN-α2b-induced apoptotic pathway monitored by the caspase 8 activity, being the effect greater with the VWT protein. Both V proteins decrease the activity of caspase 9 of the intrinsic apoptotic pathway. In a system without IFN, the VWT and VGly proteins expression promotes activation of caspases 3 and 7. However, when the cellular system was stimulated with IFN-α, this activity decreased partially. TUNEL assay shows that for treatment with IFN-α and ibuprofen of cervical adenocarcinoma cells there is nuclear DNA fragmentation but the V protein expression reduces this process.ConclusionsThe reduction in the levels of caspases and DNA fragmentation, suggesting that V protein, particularly VWT protein of Urabe AM9 vaccine strain, modulates apoptosis. In addition, the VWT protein shows a protective role for cell proliferation in the presence of antiproliferative signals.
Highlights
The Urabe AM9 vaccine strain of mumps virus contains two variants of V protein: VWT and VGly
Activation of the JAK-STAT pathway by IFN simultaneously activates others processes regulated by IFN such as apoptosis, a physiological process where cells undergo morphological changes, activation of proteases, nuclear DNA fragmentation and cell death [1,2]
In the IFN-treated cells that express VWT and VGly proteins, the activity of caspase 9 decreased 35% and 38%, respectively (Figure 1B), suggesting the activation of the apoptotic mitochondrial pathway, which may be reduced by expression of V proteins of the mumps virus regulating the magnitude of apoptosis
Summary
The Urabe AM9 vaccine strain of mumps virus contains two variants of V protein: VWT (of HN-A1081 viral population) and VGly (of HN-G1081). TUNEL assay shows that for treatment with IFN-a and ibuprofen of cervical adenocarcinoma cells there is nuclear DNA fragmentation but the V protein expression reduces this process.
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