Abstract
Fractionation of extracts from prostaglandin E 1-treated cells by polyacrylamide gel electrophoresis followed by subsequent assay of the gel slices for cyclic AMP-and cyclic GMP phosphodiesterase activities reveals multiple activities, which differ from the activities demonstrable in controls, upon assay with low levels of substrate. Preparation of extracts by gentle homogenization under isotonic conditions show the absence of some cyclic AMP phosphodiesterase activities otherwise detectable in the super-natants of hormone-treated cell extracts obtained by freezing and thawing. No such adherence to particulate fractions is obvious in comparable extracts from control cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
