Abstract
Nuclei from pea root meristem cells incorporate [adenine- 14C]NAD when grown in White's medium. When the proteins from these nuclei were extracted, several proteins were modified by the isotope as determined by SDS-gel electrophoresis and fluorography, the most significant being a band which corresponds to calf-thymus histone H2-AS. The incorporation of [adenine- 14C]NAD is a prerequisite for the formation of poly(ADP-ribose). This report marks the third plant species, following wheat and tobacco, in which some plant histones were found to be modified by [adenine- 14C]NAD. The addition of the cell cycle regulator trigonelline had no effect on which proteins were being modified.
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