Abstract
A novel actinobacterium, designated strain JXJ CY 19T, was isolated from a culture mat of Microcystis aeruginosa FACHB-905 collected from Dianchi Lake, South-west China. 16S rRNA gene sequences comparison of strain JXJ CY 19T and the available sequences in the GenBank database showed that the strain was closely related to Modestobacter marinus 42H12-1T (99.1% similarity) and Modestobacter roseus KLBMP 1279T (99.0%). The isolate had meso-diaminopimelic in the cell wall with whole-cell sugars of mannose, rhamnose, ribose, glucose, galactose, and arabinose. The menaquinone detected was MK-9(H4), while the major cellular fatty acids include C17:1 ω8c, C15:0 iso, C15:1 iso G and C16:0 iso. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified phospholipid. The DNA-DNA hybridization values between strains JXJ CY 19T and the closely related type strains Modestobacter marinus CGMCC 4.5581T and Modestobacter roseus NBRC 108673T were determined to be 50.8 ± 0.8% and 44.1 ± 1.7%, respectively. The DNA G+C content was 71.9 mol%. On the basis of the above taxonomic data and differences in physiological characters from the closely related type strains, strain JXJ CY 19T was recognized as a novel species of the genus Modestobacter, for which the name Modestobacter lacusdianchii sp. nov. (JXJ CY 19T = KCTC 39600T = CPCC 204352T) is proposed. The type strain JXJ CY 19T can solubilize calcium phosphate tribasic (Ca3(PO4)2), phytin and L-α-phosphatidylcholine. The phosphate-solubilizing property of the novel actinobacterium could be a possible factor for the increase in growth of Microcystis aeruginosa FACHB-905 in ecosystem where the amount of available soluble phosphate is limited such as Dianchi Lake.
Highlights
Dianchi Lake, the largest freshwater lake in Yunnan Province and the sixth largest in China, has been heavily polluted owing to unchecked inflow of industrial, agricultural, and domestic wastes
During analysis of culture mat of Microcystis aeruginosa FACHB-905 isolated from Dianchi Lake, a phosphate-solubilizing novel actinobacterium designated strain JXJ CY 19T belonging to the genus Modestobacter was isolated
About 0.2 ml of M. aeruginosa FACHB-905 culture obtained from Freshwater Algae Culture Collection at the Institute of Hydrobiology (FACHB collection), Chinese Academy of Sciences (CAS), Wuhan, China was spread on International Streptomyces Project medium 2 (ISP 2) [14] and incubated at 28°C for 5 days
Summary
Dianchi Lake, the largest freshwater lake in Yunnan Province and the sixth largest in China, has been heavily polluted owing to unchecked inflow of industrial, agricultural, and domestic wastes. The untreated disposal of waste is the leading cause for high biochemical oxygen demand, nitrate and phosphate, thereby providing a source for increase of cyanobacterial blooms [1]. During analysis of culture mat of Microcystis aeruginosa FACHB-905 isolated from Dianchi Lake (http://algae.ihb.ac.cn/), a phosphate-solubilizing novel actinobacterium designated strain JXJ CY 19T belonging to the genus Modestobacter was isolated. This manuscript described the polyphasic characterization of this actinobacterial strain. The manuscript reports the effect on the growth of M. aeruginosa under in vitro conditions by co-culturing with the novel actinobacterial strain JXJ CY 19T
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