Abstract
Clostridium thermocellum uses cellobiose as a favored carbon source. After growth in cellobiose, cells cannot grow on glucose or fructose until they mutate to glucose or fructose utilization (1). This usually requires 180–200 h. One explanation for the initial lack of cell growth on monosaccharides is that cellobiose uptake and metabolism occur by a different phosphorylative mechanism than that for glucose or fructose, and the cells require a mutation to activate the new type of system. Our results show that there is no difference in the mode of phosphorylation of the three sugars. They all use an adenosine triphosphate-dependent mechanism rather than the phosphoenolpyruvate-phosphotransferase system.
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