Abstract
Matrix metalloproteinase 2 (MMP2) was previously reported to play important role in the process of stem cell migration and various differentiation behaviors of muscle-derived mesenchymal stem cells (M-MSCs). However, its role and regulatory mechanism in the osteoblast differentiation and calcification of M-MSCs remain unclear. In the current study, we found that MMP2 could facilitate the osteoblast differentiation and calcification of muscle-derived mesenchymal stem cells by up-regulating the expression of some typical osteogenic differentiation phenotype markers, such as runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), and osteocalcin (OCN). Besides, we further demonstrated that MMP2 could be directly targeted by miRNA-29b-3p, which was validated by dual luciferase reporter gene and rescue assays. Moreover, increased expression of MMP2 could contribute to forming more calcium nodules representative for osteoblast calcification verified by the alizarin red staining, vice versa. In conclusion, we identified the role of miRNA-29b-3p/MMP2 relationship in regulating osteoblast differentiation and calcification of M-MSCs, which may complement the effect of MMPs family on the osteoblast differentiation to some extent, providing some potential clinical application value in the promotion of bone formation in the future.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
