Abstract
Objective This study is designed to clarify that miR-1258 targets E2F1 to regulate the proliferation and cell cycle of breast cancer (BC) cells and consequently suppress the progression of BC. Methods Bioinformatics analysis was used to analyze the differentially expressed genes in BC. The expression of miR-1258 and E2F1 mRNA in BC cell lines and immortalized breast epithelial cell lines were detected by qRT-PCR. The proliferation and growth activity of BC cells were detected by MTT and colony formation assays. The apoptosis and cell cycle of BC cells were detected by flow cytometry and the targeting relationship between miR-1258 and E2F1 was identified by dual-luciferase assay. Results The expression of miR-1258 was decreased while that of E2F1 was increased in BC cells. Overexpression of miR-1258 and silencing E2F1 could inhibit the cell proliferation and growth, block cells in the G0/G1 phase, and promote cell apoptosis. Besides, miR-1258 inhibited cell proliferation and growth, block cells in the G0/G1 phase, and promote cell apoptosis by downregulating E2F1. Conclusion miR-1258 regulates the proliferation and cell cycle to inhibit the progression of BC by targeting and downregulating E2F1.
Highlights
Breast cancer (BC) is a hormone-dependent cancer most frequently diagnosed in women, and it poses a serious threat to women’s life and health [1, 2]
MicroRNAs, small non-coding RNA molecules expressed in different tissue and cell types, are key regulators inhibiting the expression of target genes, and the dysregulation of miRNAs tends to initiate various diseases [5]. miR-1258 regulates the occurrence and development of multiple cancers, such as oral squamous cell carcinoma, liver cancer, and gastric cancer [5,6,7], and it shows a relationship with BC to some extent with its expression lowly expressed [8]
This study examined the effect of miR-1258 overexpression on BC cells, as well as predicted and validated the target gene of miR-1258 to state the mechanism of miR1258 regulating the progression of BC
Summary
This study is designed to clarify that miR-1258 targets E2F1 to regulate the proliferation and cell cycle of breast cancer (BC) cells and suppress the progression of BC. The expression of miR-1258 and E2F1 mRNA in BC cell lines and immortalized breast epithelial cell lines were detected by qRT-PCR. The apoptosis and cell cycle of BC cells were detected by flow cytometry and the targeting relationship between miR-1258 and E2F1 was identified by dual-luciferase assay. Overexpression of miR-1258 and silencing E2F1 could inhibit the cell proliferation and growth, block cells in the G0/G1 phase, and promote cell apoptosis. MiR-1258 inhibited cell proliferation and growth, block cells in the G0/G1 phase, and promote cell apoptosis by downregulating E2F1. MiR-1258 regulates the proliferation and cell cycle to inhibit the progression of BC by targeting and downregulating E2F1 Conclusion. miR-1258 regulates the proliferation and cell cycle to inhibit the progression of BC by targeting and downregulating E2F1
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