Abstract
Neural stem cells are self-renewing, multipotent and undifferentiated precursors that retain the capacity for differentiation into both glial (astrocytes and oligodendrocytes) and neuronal lineages. Neural stem cells offer cell-based therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries. However, their cellular behavior is poorly understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs involved in cell development, proliferation and differentiation through regulating gene expression at post-transcriptional level. The role of miR–381 in the development of neural stem cells remains unknown. In this study, we showed that overexpression of miR–381 promoted neural stem cells proliferation. It induced the neural stem cells differentiation to neurons and inhibited their differentiation to astrocytes. Furthermore, we identified HES1 as a direct target of miR–381 in neural stem cells. Moreover, re-expression of HES1 impaired miR-381-induced promotion of neural stem cells proliferation and induce neural stem cells differentiation to neurons. In conclusion, miR–381 played important role in neural stem cells proliferation and differentiation.
Highlights
IntroductionNeural stem cells are undifferentiated precursors, self-renewing cell populations that retain the ability to differentiate to both glial (astrocytes and oligodendrocytes)and neuronal lineages[1,2,3,4,5]
Neural stem cells are undifferentiated precursors, self-renewing cell populations that retain the ability to differentiate to both glialand neuronal lineages[1,2,3,4,5]
We demonstrated that overexpression of miR–381 promoted neural stem cells proliferation and differentiation to neurons while it inhibited their differentiation to astrocytes
Summary
Neural stem cells are undifferentiated precursors, self-renewing cell populations that retain the ability to differentiate to both glial (astrocytes and oligodendrocytes)and neuronal lineages[1,2,3,4,5]. Neural stem cells are found in the adult and developing mammalianCNS (central nervous system)[6, 7]. Recent data show that neural stem cells can serve as cell replacement therapies for neurological disorders such as Alzheimer's disease, Parkinson's disease, Huntington's disease and spinal cord injuries[8,9,10,11]. Despite the great hope of using neural stem cells for clinically intervention, it is still a long distance before clinical application of neural stem cells[12–. MiR-381 Regulates Neural Stem Cell Proliferation and Differentiation. It is urgent to understand the molecular pathways controlling NSC proliferation and differentiation
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