Abstract
miR-34 was reported to be involved in multiple tumors occurrence and development. The aim of the present study was to explore the impact of miR-34 on osteosarcoma and related mechanisms. Tumor tissues and non-tumor tissues of 34 patients with osteosarcoma were collected. qRT-PCR detection revealed that miR-34 was significantly down-regulated in tumor tissues (P<0.05). hFOB 1.19 and MG-63 cells were cultured. qRT-PCR detection showed that miR-34 was also significantly down-regulated in MG-63 cells (P<0.05). After transfection by miR-34 mimics, MG-63 cells proliferation in nude mice was significantly impaired (P<0.05), and percentage of apoptosis as well as caspase-3 positive cells proportion of osteosarcoma tissue in nude mice was markly increased (P<0.05). Western blot and immunofluorescence results also demonstrated that TGIF2 relative expression and TGIF2 positive cells proportion were both dramatically decreased (P<0.05). By luciferase reporter assay, we found that TGIF2 was the target gene of miR-34. After transfected by TGIF2 overexpression vector or co-transfected by miR-34 mimics and TGIF2 overexpression vector, we observed that, compared with blank group, tumor volume was significantly increased and apoptotic cells as well as caspase-3 positive cells proportion was obviously decreased in TGIF2 group (P<0.05), while no significant difference was found in these indicators between blank group and TGIF2 + mimics group. We concluded that miR-34 inhibited growth and promoted apoptosis of osteosarcoma in nude mice through targetting regulated TGIF2 expression.
Highlights
Osteosarcoma has a serious negative impact on bone growth and often occurs in children and adolescents [1]
Down-regulation of miR-34 in osteosarcoma tissues and cells miR-34 expression in tumor tissues and non-tumor tissues of patients with osteosarcoma was determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR)
From 1 to 6 weeks, there was no significant difference in tumor volume between blank group and negative control (NC) group (Figure 2B)
Summary
Osteosarcoma has a serious negative impact on bone growth and often occurs in children and adolescents [1]. It is often found in long bones and mainly occurs in the knee, with an incidence of 4.4 per million people worldwide [2]. The 5-year survival rate is no more than 30% for osteosarcoma patients with recurrent or metastasis [3]. Over the past 30 years, improved prognosis of osteosarcoma has been achieved gradually [4]. The recurrence and metastasis rates remained high. There is an urgent need to find more effective therapy method for the treatment of osteosarcoma
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