Abstract
MicroRNAs (miRNAs) have been suggested to play a vital role in regulate tumor progression and invasion. However, the expression of miR-339-5p in colorectal cancer and its effects are not known. Here, we report that miR-339-5p is a tumor suppressor by regulating expression of PRL-1. In this study, we showed that downregulated miR-339-5p levels in colorectal cancer tissues and highly invasive CRC cell lines. Furthermore, enhancing the expression of miR-339-5p inhibited CRC cell growth, migration and invasion in vitro and suppressed tumor growth in vivo. We then screened and identified a novel miR-339-5p target, phosphatases of regenerating liver-1 1 (PRL-1), and it was further confirmed by luciferase assay. Overexpression of miR-339-5p would also reduce the expression of PRL-1 mRNA and protein. The reduced PRL-1 expression was associated with low expression of phosphorylated-extracellular signal-regulatedkinase1/2 (p-ERK1/2). Conversely, reduction of miR-339-5p by inhibitors in cells stimulated these phenotypes. In conclusion, our results demonstrate that miR-339-5p functions as a tumor suppressor and plays a role in inhibiting growth and metastasis of CRC cells through targeting PRL-1 and regulating p-ERK1/2 .These findings suggest that miR-339-5p may be useful as a new potential therapeutic target for CRC.
Highlights
Colorectal cancer is one of the most prevalent carcinomas throughout the world
To study the expression pattern of miR-339-5p, we examined miR-339-5p mRNA expression in 20 colon cancer tissues and their pair-matched adjacent normal colonic tissues using real-time quantitative reverse transcriptase-PCR (RT–PCR)
We detected the expression of miR339-5p in six human colonic carcinoma cell lines by real-time quantitative reverse transcriptase-PCR (RT–PCR) and found that there are differences in the levels of miR-339-5p mRNA existing among the six human colon cancer cell lines
Summary
Colorectal cancer is one of the most prevalent carcinomas throughout the world. More than 1 million individuals will develop colorectal cancer, and the disease-specific mortality is nearly 33% in the developed world [1]. The depth of tumor progression and migration has been acknowledged as major prognostic factors in CRC patients [2]. The progression of this disease undergoes many decades and involves multi-step genetic events [3]. The molecular mechanisms underlying this process still can not be documented [4]. With the development of advanced genomic technology, a newly discovered class of noncoding small RNA, termed miRNAs, have attracted enormous interest in colon cancer research [5]
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