Abstract
Excessive fat deposition in the hepatocytes, associated with excess dietary fat intake, was related to the occurrence of fatty livers in fish. miR-101b plays the important roles in controlling lipid metabolism, but the underlying mechanism at the post-transcriptional level remains unclear. The purpose of this study is to explore the roles and mechanism of miR-101b-mediating lipid deposition and metabolism in yellow catfish Pelteobagrus fulvidraco. We found that miR-101b directly targeted fatty acid translocase (cd36), caspase9 (casp9) and autophagy-related gene 4A (atg4a). Furthermore, using palmitic acid (PA) or oleic acid (OA) to incubate the primary hepatocytes of yellow catfish, we demonstrated that miR-101b inversely regulated cd36, casp9, and atg4a expression at the transcriptional level; the inhibition of miR-101b aggravated fatty acids (FAs, PA or OA)-induced lipid accumulation, indicating that miR-101b mediated FAs-induced variations of lipid metabolism in yellow catfish. Taken together, our study gave novel insight into the regulatory mechanism of lipid deposition and metabolism and might provide potential targets for the prevention and treatment of fatty livers in fish.
Highlights
At present, excessive hepatic lipid deposition and fatty livers are a common phenomenon for fish under the intensive aquaculture
Accumulating evidences have shown that miRNAs are implicated in the lipid metabolism [14,15,24], suggesting that miRNAs may be a novel therapeutic target to fatty livers
We found that miR-101b directly targeted cd36, casp9 and atg4a by using reporter gene analysis
Summary
Excessive hepatic lipid deposition and fatty livers are a common phenomenon for fish under the intensive aquaculture. Metabolism, and their regulatory processes are very complex, which involves in the balance among lipid absorption, transport, lipogenesis, and lipolysis, and several crucial enzymes and transcriptional factors participate in these metabolic processes [2]. Accumulating evidences suggest apoptosis and autophagy are the pivotal pathways in regulating lipid metabolism [5,6]. Casp and ATG4 are the pivotal proteins involved in the apoptosis and autophagy pathways, respectively, which contributes to the control of lipid metabolism [7,8,9].
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