Abstract
Duku (Lansium domesticum) has a high diversity. There are no specific markers to assess genetic diversity in Duku. Microsatellite markers can determine genetic diversity more specifically in the cultivar level. This study aimed to isolate and characterize microsatellite sequences from the assembled-genome database. The markers were developed from 455 010 contigs of the velvet-assembled genome using the MISA program. BLASTN program was used for sequence annotation.Characterization of microsatellite markers was analyzed using bioinformatics. The result was found 7 types of repeat type: 63 contigs of mononucleotide with most motif of T, 450 contigs of dinucleotide with most motif of TA, 3094 contigs of trinucleotide with most motif of AAT, 561 contigs of tetranucleotide with most motif of TTAA, 85 contigs of pentanucleotide with most motif of TTTCT, 144 contigs of hexanucleotide with most motif of AAAAAT and 1331 contigs of compound sequence. This study provides information about specific markers in L. domesticum and will contribute to plant breeding development.
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