Abstract
DNA hybridization method is one of the most important techniques for detecting new disease genes. However, temperature cross-sensitivity is ubiquitously found in the DNA hybridization process, which may cause DNA damage and even produce false positives. An optical DNA hybridization sensor with temperature compensation based on dual surface plasmon resonance (SPR) effect by using a gold nanofilm coating optical fiber was proposed and demonstrated. Two parameters of DNA and temperature detection were realized by different functional modifications of the gold nanofilm. And a matrix equation was employed to analyze the relationship between two variables. Lung cancer-related genes exon-20 gene fragment was successfully detected in our work. From the theoretical and experimental results, the DNA detection limit was 9 nmol/L, and the temperature sensitivity reaches -1.74 nm/°C. The designed sensor could detect DNA with low concentration and measure temperature at the same time. The proposed sensor has the potential to be applied in disease marker gene detection with temperature compensation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
