Abstract
Pseudomonas aeruginosa CLRI BL22 was able to degrade metanilic acid, a sulfonated aromatic amine upto a concentration of 1,500mgl(-1). The degradation of metanilic acid started when the organism reached its exponential growth phase. Chromatographic and spectrophotometric analyses confirmed the presence of aniline and β-keto adipic acid indicating the ortho pathway reaction. Further proof for this pathway was provided by dioxygenase activity of the strain grown in the medium containing metanilic acid and glucose.
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