Mineral Dust Promotes Alterations in Ferritin Isoforms and Oxidative Damage to Proteins in Rat and Human Alveolar Macrophages

Abstract

Abstract The disposition of iron in the alveolar macrophage (AM) may be a critical factor in its reactivity to inhaled mineral dust. Dust particles such as coal and silica contain leachable iron or iron oxides on their surface which may become available as a catalyst for the generation of free radicals by the activated AM. This process may be regulated by iron storage proteins, which play an important role in the intracellular availability of iron. To characterize the relative effects of coal and silica dust on the content of the principal iron storage protein in the AM, we exposed human and rat AM to either respirable coal or silica dust (100 μg/ml) and evaluated the levels of intracellular ferritin. Analysis of ferritin isoforms (H- and L-chain) using monoclonal antibodies revealed a ratio of H- to L-isoforms of 1.6/1 in untreated human AM. Exposure of human AM to coal dust for 24 hours resulted in a 2.2-fold increase in the H-isoform and a 1.4-fold increase in the L-isoform. In contrast, silica dust ex...