Mind Bunyan Cards: Innovation in Arabic Learning Media to Improve Simple Sentences in Writing Skill

The Effectiveness of a Human Patient Simulator in the ATLS Shock Skills Station

Objectives • To assess the intensity of labor pain and outcome of labor among the control group. • To assess the effectiveness of ambulation on intensity of labor pain and outcome of labor among the experimental group. • To assess the usefulness of ambulation by opinionnaire among experimental group. • To compare the effectiveness of ambulation on the intensity of labor pain and outcome of labor among experimental and control groups. • To find an association between the intensity of labor pain with selected demographic variables. Materials and methods An experimental research approach was used for the study. Random sampling technique was used to allocate the subjects into experimental and control groups. Visual analog scale was used to assess the intensity of pain and observational check list was used to assess the outcome of labor. The study comprised of 40 primigravida mothers and they were grouped as experimental and control through randomization. One group received ambulation treatment, the other did not. The researcher then observed the groups to determine the effect of the treatment. Results The collected data was analyzed by descriptive and inferential statistics. The intensity of pain revealed that 40% in the experimental and 55% in the control groups experienced severe pain. The labor augmentation revealed that both oxytocin and cerviprime gel was used for 30% in the experimental and 35% in the control groups. On the mode of delivery, majority of mothers (60%) had normal vaginal delivery both in experimental and control groups. On the duration of the first stage of labor revealed that 50% in the experimental and 20% in the control groups were between 10 to 12 hours. Second stage of labor revealed that majority were between 1 and 2 hours in both the control and experimental groups. The opinion of experimental group mothers on ambulation revealed that half of the mothers (50%) agreed for recommending the ambulation for their neighbors and friends and less than half (35%) of them have agreed for comfort during first stage of labor and 40% of them have agreed for ambulation should be made as a routine in labor room. In the experimental group, mean pain score (6.8) is less than the control group (7.5). The calculated Mann-Whitney Z-value (2.045) is greater than the Z α-value of 1.960 at 0.05 level of significance. In the experimental group, the mean duration of first stage of labor score (3.95) is significantly higher than the control group mean score (3.00). The mean outcome of labor in experimental group (13.95) is greater than the control group. Conclusion There was a significant difference in the duration of first stage of labor between the experimental and control groups. But, there was no significant difference in the overall outcome of labor between experimental and control groups. There was no significant association between intensity of labor pain with selected demographic variables. This may be because of small sample size. How to cite this article Savitha V, Nayak S, Paul S. Effect of Ambulation during First Stage of Labor on Labor Pain and Outcome of Labor among the Primigravida Mothers in a Selected Hospital, Mangalore. J South Asian Feder Obst Gynae 2013;5(1):1-3.

Objective To investigate the effects of probiotics on the survival of sepsis mice and their mechanism. Methods A total of 60 male C57BL6 mice were randomly divided into 3 groups, respectively, the sham operation group (n = 20), the control group (n = 20) and the experimental group (n = 20). After feeding 2 d, mice in the experimental group were given 200 μL probiotics solution daily; mice in the control and sham operation groups were given 200 μL NaCl solution, with continuous intragastric administration for 4 weeks. Cecal ligation and puncture (CLP) was performed on the mice in the experimental and control groups, while mice in the sham operation group were performed with the same procedures of CLP except for cecal puncture. Ten mice in each group were used to observe their activity and survival of 7 d, and the blood and colon tissues were taken in the other 10 mice in each group after 24 hours. The expression levels of serum inflammatory factor interleukin 22 (IL-22), IL-2 and tumor necrosis factor alpha (TNF-α) were detected by enzyme-linked immunosorbent assay, the colon tissues were measured by the hematoxylin and eosin (HE) staining method, and the expression of colonic mucosa (Occludin) in each group was observed by the immunohistochemical method. Results Mice in the sham operation group grew well, mice in the control group curled up in a corner of the cage and trembled, and mice in the experimental group were more active than the experimental group, without obvious tremor. At 7 d, there were still 3 survived mice in the experimental group which were given euthanasia; the 7 d survival was significantly higher than that of the control group (P = 0.020). At 7 d, 10 mice in the sham operation group all survived and were given euthanasia. The expressions of serum IL-22 [(103 ± 23) ng/L vs. (27 ± 9) ng/L, t = 7.590, P < 0.001], IL-2 [(328 ± 27) ng/L vs. (77 ± 21) ng/L, t = 21.368, P < 0.001] and TNF-α [(94 ± 22) ng/L vs. (56 ± 9) ng/L, t = 4.734, P < 0.001] in the control group were significantly different as compared to the sham operation group. Meanwhile, the expressions of serum IL-22 [(75 ± 33) ng/L vs. (27 ± 9) ng/L, t = 3.755, P = 0.001], IL-2 [(217 ± 30) ng/L vs. (77 ± 21) ng/L, t = 10.850, P < 0.001] and TNF-α [(107 ± 20) ng/L vs. (56 ± 9) ng/L, t = 5.956, P < 0.001] in the experimental and sham operation groups all showed statistically significant differences. Compared with the control group, the expressions of serum IL-22 [(103 ± 23) ng/L vs. (75 ± 33) ng/L, t = 2.185, P = 0.042] and IL-2 [(328 ± 27) ng/L vs. (217 ± 30) ng/L, t = 8.371, P < 0.001] in the experimental group were significantly different, while the TNF-α [(94 ± 22) ng/L vs. (107 ± 20) ng/L, t = 1.363, P = 0.188] expression showed no statistically significant difference. In the sham operation group, the colonic mucosa was intact and the glands were regularly arranged with little or no inflammatory cell infiltration. However, mice in the control group appeared derangement, deformation and lack of colonic mucosal epithelial glands, fuzzy connected structures of enterocytes, and extensive infiltration of inflammatory cells some of which had crypt abscess. The colonic epithelia of mice in the experimental group were basically complete without erosion and loss, glands were normally arranged, and the infiltration of inflammatory cells decreased more as compared to the control group. The results of immunohistochemistry showed that mice in the sham operation group had complete acinar structures of colonic epithelial cells and more Occludin proteins. The acinar structures of colonic epithelial cells in the control group were destructed and disappeared, and there were infiltration of inflammatory cells and less Occludin proteins. The acinar structures of colonic epithelial cells in the experimental group were complete with widened interacinar gaps, and the expression of Occludin protein increased as compared to the control group. Conclusion Probiotics can inhibit the reduction of Occludin of intestinal epithelial cells and stabilize barrier structures of intestinal mucosas, thus effectively improving the survival of septic mice. Key words: Probiotics; Sepsis; Occludin

This randomized-controlled trial demonstrates that an exercise consultation, based on the transtheoretic model of exercise behavior change, significantly improves short-term adherence to exercise.

Zmiany metylacji i oksydacji w próbkach DNA bydła bytującego w środowisku zanieczyszczonym metalami ciężkimi

The instructional-educational process in learning to swim plays a crucial role in children's development. Approaching methods and use in training practices of all ages and performance levels have been the subject of ever deeper research, so that the effectiveness of training in this area is increasingly visible. The issue of motivation in sports is a topical issue because it is essential for athletes' involvement and performance. Although material stimulation can be considered an important factor in motivating athletes, it is not necessarily enough to commit them to an extremely difficult and risky path, such as that of performance sports. Athletes' motivation stems from a complex combination of factors, including passion for sport, the desire to demonstrate their skills and talents, the aspiration to achieve personal and collective goals, and the desire to achieve recognition and satisfaction through sporting success. The purpose of the research is to identify the level of motivation for swimming and performance of children aged 9-10 years. Research method: the test Motivation for swimming of children aged 9-10 years (adaptation after N. Luskanova) The test was applied by the researcher in October 2022 to a number of 30 children aged 9-10 who practice swimming in the Tirgoviște Swimming Complex and CSȘ Tirgoviște Swimming Pool. Depending on age, we have, within the experimental group, 7 children aged 9 and 8 children aged 10. Within the control group, we identify 6 children aged 9 years and 9 children aged 10 years. We observe that the two groups are homogeneous, referring to the age variable. Regarding the gender of the subjects who are part of the group subjected to the experiment, we observe homogeneity in terms of gender, in each group there are 5 girls and 10 boys. The distribution according to the residential environment from which they come is as follows: from the experimental group 3 subjects come from the rural environment and 12 from the urban environment, and in the control group 1 subject comes from the rural environment and 14 from the urban environment The test results can be seen in Table 1. The difference between the values obtained between the experimental and control groups is not significant from a statistical point of view, since the values of the statistical indicators are close, we observe small differences between them.Table 1. Distribution of results on the Motivation for swimming test of children aged 9-10 years (adaptation according to N. Luskanova), experimental group and control group (n=20 boys)tabelThe differences between the two groups in the statistical indicators are variable, the difference between the averages is small, 0.6 points, in the standard deviation the difference between the groups is 0.77 points, the minimums recorded are equal to 15 points, and the difference between the maximums of the groups is 1 point. These results show homogeneity regarding the level of motivation between the experimental group and that of the control group. Table 2. Analysis of the level of motivation for swimming in the experimental and control groups, boystabelWe find that the level of motivation for swimming of the interviewed subjects is good. On the first level are ranked 2 subjects from the experimental group SC with 26 points and CR with 25 points, and from the control group subject MR with 25 points. These subjects have a very good motivation to practice swimming. On the second level, a number of 3 subjects from experimental group II are ranked with 24 points, AN with 23 points, AT with 20 points and six control subjects BM with 24 points, NA and RN with 23 points each, CI, AI and VE with 21 points each. The motivation of these subjects is characterized as good. Level III, respectively of relatively good motivation, has 5 subjects from the experimental group in the ranking: DF with 19 points, MG with 18 points, BL and IA with 17 points, GI with 15 points. In the control group, three subjects were registered on this level: BG with 19 points, RO with 18 points and DC with 15 points. None of the subjects was ranked on level IV-weak motivation and on level 5-very weak motivation. Overall, the motivation of 9-10 year olds for swimming is good and relatively good. In the following we will analyze the results recorded by the girls' teams. Table 3. Distribution of results on the Motivation for swimming test of children aged 9-10 years (adaptation according to N. Luskanova), experimental group and control group, (n= 5 girls)tabelThe difference between the values obtained between the experimental and control groups is not statistically significant as the values of the statistical indicators are close in value. The difference between the averages of the two groups is 1.04 points, the standard deviation being 2.82 points in the case of the experimental group and 1.49 in the case of the control group. The difference between the minimum values is 1 point, and between the maximum values is 4 points in favor of the experimental group. The results obtained by the girls from the two groups subjected to the experiment, in the Motivation for swimming test of children aged 9-10 years, are as follows: on the first level, with a very good motivation, only GM ranked with 26 points, from the experimental group , on level II, with good motivation, the subjects AF with 22 points and BM with 20 points from the experimental group and DV with 22 points, AN and IF with 20 points each from the control group are highlighted. On level III, relatively good motivation, there are EL with 19 points and MT with 18 points from the experimental group and BP and NO with 18 points each from the control group. Table 4 Analysis of the level of motivation for swimming in the experimental and control groups, girlstabelConclusions The analysis of the obtained data shows us that both groups subjected to the experiment, both in the case of boys and girls, have a good motivation for swimming, but there is a need to increase the level of motivation to maintain interest in this sport and to register performance, through specific activities. We believe that an increased level of motivation among children between the ages of 9 and 10 in swimming practice can have a significant impact on their development both in sports and personally. In correlation with the research results, we propose the following recommendations: - For each group of children, by age level, a psychological training program targeting the motivational component will be developed, which will be integrated into the training process. This program will be adapted to the specific needs of the group and will aim to adapt motivation and, implicitly, sports performance. - The psychological training program will include strategies and techniques to support the development of children's motivation for swimming, promoting commitment, the desire to excel and perseverance in training and competitions. This could include, among other things, visualization sessions, stability of personal goals, working with thoughts and emotions to maintain a positive mindset and focus on success.

Various games and activities of students can be used as an effective tool in the development of mental capacities, skills and intelligence games. Intelligence games are games that have all kinds of problems, including real problems. So it is a good tool to teach problem solving. The course of intelligence games will enable students to develop capacity for problem perception and assessment, to create different perspectives, to be able to make quick and correct decisions when they encounter problems, to develop a problem-solving and problem-solving habit, and to use reasoning and logic effectively. Therefore, it is appropriate to use a stepwise teaching approach to teaching mental games. In the middle school the course of intelligence games teaching program, learning areas are divided into 6 categories according to game categories: Reason Execution and Transaction Games, Verbal Games, Geometric - Mechanics Games, Strategy Games, Memory Games and Intelligence Questions [1]. The purpose of this research is to examine the effects of the course of intelligence games on the mathematics attitudes of Grade 5 students. Research was conducted in the 5th Grade of a private school in Antalya province. The experimental part of the research is comprised of the effects of the course of intelligence on students’ mathematics attitudes. This part of the research is conducted by the pretest-post-test control group design. Of two equal classes, one group is randomly assigned to be the experimental group and the other the control group; pre-test and post-test measurements were carried out in both groups. Experimental design with control group was used in the research. Mathematics attitude scale was used as pre-test and post-test in the research. In the analysis of the data, t test was used to compare the pretest and posttest scores. Between the pretest and the posttest, the lectures in the experimental group were carried out during the education period, including 2 hours per week. The lessons are supported each week by different activities of mental games. In the control group, teaching was done according to the current curriculum. To determine whether there is a meaningful difference between pre-test and post-test scores of experiment and control groups, analyzes were tried to determine the effect of mental games on math attitude. According to the results of the analysis, it was found that the students in the experimental and control groups had a significant difference between their pre-test scores and post-test scores within the mathematics attitude scale.

In this study, it was investigated that effect of games including physical activity on digital game addiction of 11-14 age group middle-school students. Along with the determination of digital game addiction, conducting applied experimental study is important regarding showing results of application with theoretical knowledge towards solving problem. Within this framework, it was thought that “games including physical activity” will be important tool for decreasing the level of digital game addiction. Experimental Design method was used in the study in which was adopted Quantitative Research model. When 11-14 age group students who addicted digital games consisted of the population of the study, the sample of the study consisted of 29 girls and 51 boys who have studied. In the study, personal information form and digital game addiction scale that was developed by the researcher were used as measurement tools. During data collection period, by accepting applied pre-test to students as criterion sample process, students who have high addiction level (total scale score is 49 and above) were detected and control (n = 40) and experimental (n = 40) groups were created with unbiased assignment method. After the 12-weeks period “games including physical activity” training, the data which was collected from the control and experimental groups by taking post-test data was evaluated with SPSS 20 packaged software. It was found that there was a significant difference between pre-test and post-test of both sub-dimensions and total addiction scores and post-test scores were lower than pre-test scores. It was shown that post-test addiction scores were significantly higher than pre-test addiction scores in control group. When investigating post-test addiction scores of control and experiment groups, it was shown that addiction level of experimental group significantly decrease comparing control group. It was determined that pre-test/post-test scores of experimental group students did not show significant differences in terms of gender, age, education level of mothers, education level of fathers, number of siblings. It was also found that pre-test/post-test addiction scores of experimental and control groups showed significant differences regarding playing digital game duration. When playing digital game duration decreased in experimental group, playing game duration increased in control group. Therefore, it was concluded that addiction levels of students who are digital game addict decreased because of interesting games including physical activity. As a result; physical moving games and sports can be said to be an important tool in dealing with digital game addiction. It can also be said that an important way to protect children from such addictions is to do sports.

A study of two 100-person groups of juvenile delinquents born in 1959 was conducted in the years 1981‒1985 at the Department of Criminology, Institute of Legal Studies, Polish Academy of Sciences. The first (experimental) group consisted of boys randomly selected from the total of 225 juveniles born in 1959 who had committed at least on offence while intoxicated. The other (control) group were 100 randomly juveniles selected from the entire population of 8196 juvenile delinquents born in 1959. None of the juveniles selected for the experimental group happened to find themselves in the control group as well. As shown by the findings, the juveniles who had committed at least one offence while intoxicated were much more demoralized as a group than the whole of juvenile delinquents. It seemed interesting, therefore, to follow the further fates of both groups as adults. The follow-up period was 7 years; until that time, all of the examined persons reached the age of 25 when the average Polish man be- comes stabilized to some extent, having graduated from university, worked for several or a dozen years (upon completion of secondary or elementary education respectively), and frequently having also established a family. Data on the life situation of the young men from both examined groups on their 25th birthday were obtained from the following four sources: ‒ the Central Register of Convicted Persons kept by the Ministry of Justice, and the Register of Convicted and Detained Persons where criminal records of the entire sample were checked; ‒ files of criminal cases of all men with criminal records (47.0% of the experimental and 35.0% of the control group); the files concerned criminal proceedings before common courts for offences committed after coming of age; ‒ questionnaire survey of 63.0% of the experimental and 66.0% of the control group; ‒ inquiry submitted to the sobering-up stations concerning the entire sample. As shown by the findings, 24.0% of the experimental and 13.0% of the control group established their own families before the age of 25. The proportions are high, as regards the experimental group in particular: erly in the 1980s, the newly married constituted about 10% of the total male population aged 20‒24 in Poland. The mean educational level was higher in the control compared to the experimental group; this concerns first and foremost cases of education higher than the elmementary technical (of which there were two in the experimental compared to ten in the control group). Moreover, no cases of illiteracy could be found in the control group, compared to one such case in the experimental group. Of all the men of the experimental group concerning whom data could be obtained, 80.4% had a regular job, and 19.6% stayed out of job or worked casually. Of the control group, 80.0% had a regular job (33% combining job with school), 18.3% stayed out of job or worked casually, and one person had entered university. The number of convicted persons in the experimental group (47) was larger compared to the control group (35) by 12.0%, the difference being significant. Also relapse into crime was higher in the expenmental group (l5 vs. 11 cases). The first offence committed by those convicted as young adults was mainly one against property: 35 cases in the experimental group (66.0% of all those convicted) and 28 cases in the control group (80.0%). The second most frequent offence of members of the experimental group was an aggressive act: against life and health, freedom, personal dignity and inviolability (10 persons, i.e. 18.8% of all those convicted). The offences of this type included: bodily injury (Art. 156 of the penal code – 3 persons, i.e. 6.4%); participation in a brawl or beating (Art. 158 and 159 – 1 person, i.e. 2.1%); infringement of bodily inviolability (Art. 182 – 3 persons, i.e. 6.4%); assault against a public functionary (Art. 233 and 234 – 1 person, i.e. 2.1%); insult against a public functionary (Art. 236 – 1 person, i.e. 2.1%). In the control group, 6 cases of such offences could be found (9.1% of all those convicted); yet the only offence under Art. 148 1 of the penal code, that is homicide, had been committed by a member of that goup.The other discussed figures and proportions were respectively: Art. l58, 159 – 3 persons, i.e. 8.6%; Art. 182 – 1 person (2.9%); Art. 233, 234 – 1 person (2.9%); and Art. 236 – 1 person (29%). Beside offences, the two groups manifested also other synptoms of social maladjustment. The symptoms found most often in both groups were: “contacts with persons known to the police as delinquent” and stays at the sobering-up station. As regards the experimental group, the third frequent symptom were brawls in the place of residence followed by bad opinion with neighbors, hooliganism, and avoidance of work. In the control group, avoidance of work ranked third, followed by bad opinion with neighbors, hooliganism, and brawls at the place of residence. This ranking of frequency of the symptoms of social maladjustment points to a greater aggressiveness of the young men from the experimental group. On the 63 young men from the experimental group concerning whom data could be obtained, 62 (98.0%) drank alcohol. In the control group 59 (89.0%) of the 66 concerning whom data could be obtained were drinkers. The group of drinkers included all those who had drunk several times a week already as juveniles, and 70% of those who had drunk once a week. In the control group, drinkers included 91.7% of those who had drunk as juvoniles (11 of 12 cases). Of those who had drunk as juveniles in the control group, 66.7% (8 cases) were convicted as adults. As shown by the discussed data, young men from the experimental group – those who committed as juveniles at least one offence while intoxicated prove much inferior in terms of the social situation to other men who also committed offences as juveniles but did not drink alcohol. Therefore, early alcohol consumption among juvenile delinquents is an important factor of a negative prognosis as to the further fates of such persons. A number of postulates have been formulated, addressed at the prosecuting agencies, criminal justice, and institutions designed to assist persons in extraordinary situation. With respect to the present sample, all such postulates acquire special importance and must be met without fail. Niniejszy artykuł stanowi kontynuację opracowania: Nieletni sprawcy przestępstw popełnionych pod wpływem alkoholu, „Archiwum Kryminologii” 1991, t. XVIII.

This study was designed to assess the impact of problem based learning on students’ critical thinking in science at elementary level. Major objectives of the study were to examine critical thinking’s of the students, to practice problem based learning through activities, and to evaluate critical thinking of the students after problem based activities. The study population was encompassed all students of 8th grade (20172) within District Muzaffar Garh. Sample consisted of total 50 students from within the same district. Twenty five students out of 40 were selected from Government Boys Elementary School Arshad Abad by using simple random technique as experimental group. Twenty five students out of 27 were selected from Government Boys Elementary Khara by using simple random technique as control group. The designs of study were pretest-posttest and Quasi experimental in nature. Problem based learning treatment pattern was applied. During 16-week treatment duration, the experimental group and the control group were treated with problem-based learning and taught through traditional lecture-based methods respectively. Data was collected from the control and experiment groups before and after four month problem-based learning through pre-test and posttest. After data collection, analysis of the data collected done through descriptive statistics by using mean, median, mode and standard deviation (SD) and inferential statistics by using t-test. Paired sample t-test was applied to measure significance difference value between the means of experimental and control group. T-test value is greater between experimental group and control groups in pretest than the significance value that shows there is significance statistical difference between experimental group and control group performances in pretest. Performance of experimental group is better. Value of T-test is greater between experimental and control groups in posttest than the significance value shows there is significance statistical difference in experimental and control groups performances in posttest. Experimental group performance is better. The experimental group performed better as compared to performance of control group in both pretest and posttest. But the difference in means of pretest of experimental and control group is very small than difference in means of posttest of experimental group and control groups. It depicts through problem based learning students’ critical thinking is highly improved.

Background and Objectives: Nutrition is the science of food and its relationship to health. It is concerned primarily with the part played by nutrients in body growth, development and maintains. When the person was unable to take oral feeding. Nasogastric tube feeding is a common method of maintaining or improving nutritional status in patients who are unable to take sufficient nutrition orally. Nasogastric tube feeding is a basic nursing care in caring the patients. Nurses play a vital role in care of the patients with nasogastric tube feeding. Nurses are responsible to take care of nasogastric tube and she must feed the patient properly. And also they must have adequate knowledge and better practice in order to bring positive outcome of the patient. The objectives of the study To assess the level of knowledge and practice regarding NGTF among experimental and control group in terms of pretest score. To develop and implement VAT on NGTF to the experimental group. To assess the posttest knowledge and practice scores between experimental and control group, to evaluate the effectiveness of video assisted teaching. To find out the association between the knowledge and practice scores with their selected demographic variables. Method: A study was an evaluative approach with pretest-posttest control group design.60 staff nurses were selected for the study using purposive sampling technique and distributed equally to the experimental and control group. The data was collected by using structured questionnaire and observational checklist on NGTF. The study was conducted in two hospitals of Hassan. Results: The findings of the study indicated that over all mean pretest knowledge and practice score was 40.20% and 46.38% in experimental group and 38.50% and 46.0% in control group respectively. The mean posttest knowledge and practice in experimental group was 87.60% and 90.36%, where as in control group was 40% and 40.80% and the independent ‘t’ value was 15.7 in knowledge aspect and 15.3 in practice aspect when p<0.001. There was significantly increase in the level of knowledge and practice in experimental group after the VAT. Hence the finding signifies that the VAT was effective in enhancing the knowledge and practice of staff nurses. Interpretation and conclusion: Both descriptive and inferential statistics was employed to analyze the data. The data analysis was carried out on the basis of objectives and hypothesis of the study and has been presented on the sample characteristics with knowledge and practice level. Overall mean pretest knowledge and practice score was 40.20% and 46.38% in experimental group and 38.50% and 46% in control group. The mean posttest knowledge and practice score in experimental group was 87.60% and 90.36% and in control group 40% and 40.80% respectively. Knowledge and practice of staff nurses regarding NGTF was less than 50% before the administration of VAT in both the groups. And it was more than 50% in experimental group after the administration of VAT. There was no association between knowledge and practice level with selected demographic variables. The independent‘t’ value (15.7 in knowledge and 15.3 in practice) was greater than the table value when p<0.001 level of significance. This indicated that VAT was significantly effective in increasing the knowledge and practice level of staff nurses.

We aim to use the ovariectomized cynomolgus monkey to observe the effect of long-term changes of sex hormone levels on ocular surface. Six healthy adolescent cynomolgus monkeys were randomly assigned to control and experimental groups (n=3/group). Bilateral ovariectomy was performed in experimental group, and sham operation was performed on control animals with their ovaries reserved. The concentrations of serum estradiol and testosterone in cynomolgus monkeys' lower-limb venous were detected by radioimmunoassay before surgery, and at 6, 12, 18, and 24 months after the surgery. Phenol red cotton thread test, tear osmolarity, corneal fluorescein staining, and conjunctival impression cytology were performed to evaluate the ocular surface conditions before surgery, and at 6, 12, 18, and 24 months after the surgery. The histological examinations of the bulbar conjunctiva and labial glands tissues were performed at 12 and 24 months after the surgery. There were no significant differences in the serum level of estradiol and testosterone between the experimental and control groups before surgery, at 6, and 12 months after surgery (P>0.05). Serum level of estradiol and testosterone were significantly less in experimental versus control group at 18 and 24 months after surgery (P<0.05). There was no significant differences in phenol red cotton between the experimental and control groups before surgery, at 6, 12, 18, and 24 months after surgery (P>0.05). Tear osmolarity had no significant differences between experimental and control group before surgery (P>0.05), and it was significantly higher in experimental versus control group at all four time-points after surgery (P<0.05). The number of conjunctival goblet cells had no significant differences between experimental and control group before surgery, at 6 months after surgery (P>0.05), and it was significantly lower in experimental versus control group at 12, 18, and 24 months after surgery (P<0.05). There were no significant differences in the corneal fluorescein staining score between the experimental and control groups before surgery, at 6, 12, and 18 months after surgery (P>0.05), and it was higher in experimental than control group at 24 months after surgery (P<0.05). In addition, inflammatory cell infiltration in bulbar conjunctiva and labial glands tissues was observed in experimental group and was unobserved in control group at 24 months after surgery. Ovariectomy led to decreased sex hormone levels in cynomolgus monkey. In turn, tear osmolarity was increased, the number of conjunctival goblet cells were decreased, the corneal fluorescence staining was increased, and the pathological examination of the bulbar conjunctiva and labial glands tissues were abnormal, phenol red cotton thread test did not change significantly.

Improving Primary School Students’ Creative Writing and Social-Emotional Learning Skills through Collaborative Digital Storytelling

Introduction: Aldehyde dehydrogenases (ALDH), particularly ALDH1B1 has been shown to have increased expression in both human and APCMin mice colorectal tumors. Studies have proposed the use of ALDH1B1 as a potential novel human colorectal tumor marker. Previous in vitro studies have shown that ALDH1B1 plays a critical role in colon carcinogenesis. The goal of this study is to understand the in vivo role of ALDH1B1 in colon carcinogenesis using a newly developed mouse model, APCMinAldh1b1−/− mice, through characterization of its phenotype in tumor growth and development, and elucidating the underlying potential molecular mechanisms. Methods: We generated APCMinAldh1b1−/-. At 12-14 weeks old, the experimental group APCMinAldh1b1−/- (n=6) and control group APCMinAldh1b1+/+ (n=7) were euthanized. Tumor counts, size and burden for each intestinal segments were recorded. H&amp;E staining was performed and observed for tumor types and counts. Immunohistochemistry using the markers Ki-67 (proliferation), Cleave Caspase 3 (apoptosis), KRT20 (differentiation) were done in colon. IHC of retinoic acid binding proteins (anti-CRBP2 and anti-FABP5) were also performed Results: The experimental group had shown significantly higher total average intestinal tumor burden (6.8 fold) (p=0.0007) and total average tumor counts observed (3.6 fold) (p=0.02). In the colon, there was a 3.5-fold increase in average tumor burden (p=0.017) with a 2.7-fold increase in average tumor size (p=0.025)in the experimental group. We also noticed a trend of increased average tumor counts observed in the colon of the experimental group although statistically insignificant. Average tumor counts of ileum observed was 9-fold higher in experimental group with only 1 mice from control group showed observable small tumor growth which did not meet criteria for tumor size and tumor burden calculation. There were no significant differences in observable tumor counts, tumor size and tumor burden in jejunum. Histologically, all tumors were pedunculated adenocarcinomas. Many unobservable small tumors less than 1mm width and length were seen in the H&amp;E of both ileum and jejunum in the experimental and control group. In the control group, there was less observable tumors compared to the experimental group although the tumor counts on H&amp;E in both groups showed no major differences. This indicates that the tumor size in the experimental group was larger than the control group. On the contrary, average tumor counts of colon in H&amp;E were lower compared to tumor counts observed in both control and experimental groups, indicating low number of small unobservable tumors in colon. Although the tumor counts of colon on H&amp;E and observation between the two groups were statistically insignificant, we noticed a pattern of increased tumor counts in the experimental group suggesting a potential increase in tumor incidence. Immunohistochemical staining of colon is ongoing. Conclusion: ALDH1B1 reduced tumor growth rate in the ileum, and reduced tumor size in the colon suggesting its role in tumor promotion and progression. A larger number of experimental and control group is required to establish its potentially tumor-initiating role in the colon. Further characterization of ALDH1B1 in colon carcinogenesis is ongoing. We are also elucidating the role and regulation of retinoic acid signaling by ALDH1B1 in colorectal tumor growth. Citation Format: Wan Ying Tan, David. J. Orlicky, Ying Chen, Yatrik M. Shah, David C. Thompson, Vasilis Vasiliou. Role of aldehyde dehydrogenase 1B1 in colon carcinogenesis in a newly developed mouse model [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 6141.

A study to evaluate the effectiveness of Cassia auriculata (Avaram poo) Flower extract in reducing blood glucose among pre diagnosed Type 2 Diabetes Mellitus clients in selected area of Puducherry. Objectives of the study are to assess the pretest level blood glucose among the control and experimental group. To administer the Cassia auriculata (Avaram poo) flower extract to the study group, To assess the post test level blood glucose among the control and experimental group, To evaluate the effectiveness of Cassia auriculata Flower (Avaram poo) extract in reduction of blood glucose level among type2 Diabetes Mellitus subjects, To associate the effectiveness of Cassia auricular Flower (Avaram poo) extract with demographic variables in experimental group. The research approach selected for the study was Quantitative approach, quasi Experimental design (pre-test and post test with control group). The study was conducted in selected PHC Kalapet, at Puducherry. Sample size was 60, 30 in experimental and 30 in control group were randomly assigned. The standardized glucometer was used to assess the blood glucose level. Pretest was done to assess the level blood glucose (FBS and PPBS) for both the groups. Experimental group received Cassia auriculata Flower (Avaram poo) extract for 30 days and control group did not receive any measures. Post test level of blood Glucose (FBS and PPBS) was evaluated on 30th day for both experimental and control group. The results of the study were the result revealed that the pretest mean score of fasting blood glucose was 133.50±37.43 and after the administrations of Cassia auriculata flower extract the post test mean score was 109.93±18.14. The mean difference score was 23.57. The calculated paired't' value of t =5.478 was found to statistically highly significant at p<0.,001 level. This clearly indicates that the Cassia auriculata flower extract was found to be effective in reducing the fasting blood glucose level among type 2 diabetes mellitus clients in the experimental group. The pretest mean score of post prandial blood glucose was 262.40±73.18 and after the administrations of Cassia auriculata flower extract the post test mean score was 193.43±45.99. The mean difference score was 68.97. The calculated paired 't' value of t = 8.103 was found to statistically highly significant at p<0.001 level. This clearly indicates that the Cassia auriculata flower extract was found to be effective in reducing the post prandial blood glucose level among pre diagnosed type 2 diabetes mellitus clients in the experimental group. The demographic variable of marriage had shown significant association with the post test level of fasting blood glucose among the study group.