Abstract
<p><strong>Objective: </strong>Parkinson’s disease (PD) is one of the of a neurodegenerative disorder, It’s decreased the dopaminergic neurones, tyrosine hydroxylase (TH) and increased the α-synuclein protein level. This study was conducted to investigate the neuroprotective effect of <em>Mimosa pudica</em> have the abilities to improve TH and DAT proteins expression against MPP<sup>+ </sup>induced neurotoxicity, in <em>in</em><em> vitro</em> model of<em> </em>Parkinson’s disease using SH-SY5Y human neuroblastoma cell lines. <strong></strong></p><p><strong>Methods: </strong><em>Mimosa pudica</em> were pre-treated with various concentration for cell viability assay. Vehicle alone or <em>Mimosa pudica</em> (300µg) for 24 h, and then were co-treated with 1000µM MPP<sup>+ </sup>for 15 min in the continued presence of vehicle or <em>Mimosa pudica</em>. After treatment, cells were collected for protein expression.<strong></strong></p><p><strong>Results: </strong>Cell viability assay confers the inhibitory concentration cell death of <em>Mimosa pudica</em>. MPP<sup>+</sup>significantly down-regulated the protein expression of<em> </em>TH (p&lt;0.01) and DAT (p&lt;0.05)<em>. Mimosa pudica</em> decreased the expression of α synuclein (p&lt;0.01) in MPP<sup>+ </sup>intoxicated cell lines.</p><p><strong>Conclusion: </strong>The present study showed that <em>Mimosa pudica</em> exerts neuroprotection by suppressing α synuclein and the dopaminergic neurdegeneration. <em>Mimosa pudica</em> may be due to quecertin which might be acted via the anti-oxidant mechanism. The above finding suggests that <em>Mimosa pudica</em> may act as a potential target in the management of PD. <strong></strong></p><p> </p>
Highlights
Siddha system of medicine, which is practised prevalently in the southern part of India, especially in Tamil Nadu, is familiar among Tamil-speaking people and outside of the landscape too
MPP+iodide, mouse anti-tyrosine hydroxylase (TH), mouse anti-α synuclein, rat anti-dopamine transporter (DAT), and anti-mouse IgG were purchased from Sigma-Aldrich, USA
The present study demonstrated the neuroprotective effect of Mimosa pudica against MPP+ induced neurotoxicity in SHSY5Y cell lines
Summary
Siddha system of medicine, which is practised prevalently in the southern part of India, especially in Tamil Nadu, is familiar among Tamil-speaking people and outside of the landscape too. The name Siddha medicine owes its origin to medicinal ideas and practices rendered by sages called Siddhar’s/“Holy immortals”. Siddha system of medicine is established mainly with 18 Siddhas and the most renowned are Agathiyar, Thiru moolar and Bhogar [1]. Decreased levels of dopaminergic neuronal density in the substantia nigra (SNpc) and striatum (ST) and more importantly tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis, are major biochemical indications in PD pathology. Remarkable impeachment of α-synuclein (SYN) provokes Lewy body (LB) pathologies that involve the deposition of LBs in cell bodies. Upregulation of SYN was shown to trigger the generation of TNF-α and IL–1β in cultured Neuronal cell lines [2, 3]
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