Abstract
Coronary artery disease (CAD) is one of the main causes of hospitalization worldwide and has high morbidity. MicroRNAs (miRNAs) play an important role in the pathogenesis of cardiovascular diseases. miR-451 is a special miRNA that is involved in many cancers’ development. At present, there is no research about miR-451 in coronary heart disease. In this study, we aimed to identify the action mechanism of miR-451 in coronary heart disease and human umbilical vein endothelial cells (HUVECs). In this study, we found that miR-451 is up-regulated in the peripheral blood of patients with coronary heart disease. Moreover, TargetScan and dual-luciferase reporter gene assay results showed that VEGFA is a direct target gene of miR-451. C (CCK-8) and flow cytometry assay results showed that miR-451 mimic significantly inhibits cell proliferation and promotes apoptosis in HUVECs. Moreover, we found that the role of miR-451 in HUVECs is associated with the PI3K-Akt-mTOR pathway. Taken together, the data indicates that miR-451 might be a novel bio-marker for coronary heart disease.
Highlights
Coronary artery disease (CAD) is typically caused by the development of atherosclerotic lesions and results inSo far, many epidemiological studies have demonstrated several risk factors for CAD, including dyslipidemia, diabetes, high blood pressure, smoking, and diet [6,7,8]
The results showed that miR-451b was highly expressed in the serum of patients with coronary heart disease (Figure 1A)
The results showed that miR-451b decreased the activity of the luciferase reporter fused to the 3’-UTR-WT of VEGFA but did not inhibit that of the reporter fused to the MUT version (Figure 2B)
Summary
Coronary artery disease (CAD) is typically caused by the development of atherosclerotic lesions and results inSo far, many epidemiological studies have demonstrated several risk factors for CAD, including dyslipidemia, diabetes, high blood pressure, smoking, and diet [6,7,8]. A growing body of evidence suggest that abnormal changes in the expression of some genes in CAD play a crucial role in the pathogenesis of atherosclerosis [9, 10]. MiRNAs can regulate protein translation by either complete or incomplete pairing with the target gene, or by inhibiting expression of downstream target proteins [12, 13]. Studies have shown that miRNAs are related to the development of cancer [14]. MiR-451 is a tumor suppressor gene that plays a role in tumor suppression in a variety of cancers [16]. A previous study has found that miR-451 could inhibit hepatic tumor angiogenesis, revealing that it may be involved in the formation of blood vessels [17]
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