Abstract
Purpose: To investigate the regulatory role of miR-126 in inflammation-associated gene expression in vascular smooth muscle (VSMCs) cells of diabetic rats.Methods: Diabetes was induced in rats by intraperitoneal injection of streptozocin (STZ) at a dose of 50 mg/kg. VSMCs were isolated from the aortic intimal-medial layers of the rats by a standard protocol. Expression of miR-126 was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting. Transfection was performed with Lipofectamine 2000 reagent.Results: Expression of miR-126 was significantly (p < 0.05) downregulated in the VSMCs of diabetic rats. Similarly, expressions of SOD, APX and CAT were downregulated, while those of COX, LOX and NOS were significantly upregulated in VSMCs. However, transfection-induced miR-126 overexpression in the VSMCs of diabetic rats led to significant (p < 0.05) upregulation of SOD, CAT, APX and downregulation of COX, LOX and NOS. TargetScan analysis revealed that miR-126 exerted these effects by targeting SIRT1 gene. Furthermore, qRT-PCR and western blotting revealed that miR-126 overexpression in diabetic VMSCs caused significant (p < 0.05) upregulation of the expression of SIRT-1.Conclusion: The results indicate that miR-126 regulates the expression of inflammation-related genes by targeting SIRT-1 genes in vascular smooth muscle cells of diabetic rats. Thus, miR-126 may be beneficial in the management of diabetes.Keywords: Diabetes, Inflammation, Genes, microRNA, vascular smooth muscle cells
Highlights
Diabetes is a severe metabolic and pathological condition which results in non-physiological changes in many tissues due mostly to oxidative stress [1]
Diabetic complications are associated with upregulated expressions of MicroRNAs are small non-coding RNA molecules which participate in a wide array of biological functions
The results showed that the expressions of the genes for SOD, CAT and APX were significantly downregulated in the Vascular smooth muscle cells (VSMCs) from diabetic rats, relative to VSMCs from the normal rats (p < 0.05, Figure 1 A - C)
Summary
Diabetes is a severe metabolic and pathological condition which results in non-physiological changes in many tissues due mostly to oxidative stress [1]. Factors such as increase in the population of aged people, consumption of energy-rich diets, and sedentary life-styles have led to significant increases in the number of diabetic cases globally [2]. MicroRNAs are small non-coding RNA molecules which participate in a wide array of biological functions. They regulate the expressions of most of the genes, and have been implicated in the development of several diseases and disorders [6]. Values of p
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