Abstract
MicroRNAs (miRs) are small non-coding RNAs. MiR-125b has been described as being downregulated in cataract tissue when compared to a transparent lens. The aims of the study were: 1) to establish the expression of miR-125b in cataracts complicated by pseudoexfoliation syndrome (PEX), glaucoma or PEX glaucoma; and 2) to determine whether any environmental factors influence miR-125b expression. Anterior lens capsules were obtained from 150 patients. The patients were subdivided into 1 of 4 groups: those with PEX (PEXg), those with primary open-angle glaucoma (Gg) and those with PEX glaucoma (PEXGg), plus gender-matched controls with cataracts alone (control group - Cg). Quantitative polymerase chain reaction (qPCR) expression of microRNA-125b was examined in every group. The mean age of the 150 patients was 75.18 years (standard deviation (SD) ±9.12 years). Our investigation indicated, for the first time, that miR-125b expression was increased 3.33 times in the PEXg (p = 0.015). The quantitative analysis of miR-125b expression conducted between combined groups of all the patients that have PEX syndrome (with or without glaucoma) and the Cg revealed a statistically significant difference (p = 0.04). Lower miR-125b expression was found in the patients who smoked compared to those who did not (p = 0.01). Our data revealed the possible role of miR-125b in PEX syndrome development. There are 2 possible interpretations of these results: either the co-existence of PEX acts as a moderator of miR-125b expression in the anterior lens capsule, or increased expression of miR-125b can play a role in the pathogenesis of PEX.
Highlights
The patients were selected for three groups: with pseudoexfoliation syndrome (PEX), primary open angle glaucoma, pseudoexfoliation glaucoma, with matched controls in the term of gender. qPCR expression of microRNA 125b was examined in every groups
Investigation indicates for the first time, that miR 125b expression is significantly increased 3.33x in PEX group (p=0,046)
Either the co-existence of PEX acts as a moderator of miR 125b expression in the anterior lens, or the increased expression of miR 125b can be a cause of PEX
Summary
The aim of study was [1] to establish the expression of miR 125b in cataracts complicated by pseudoexfoliation syndrome /PEX/, glaucoma or pseudoexfoliation glaucoma and [2] to determine whether any environmental factors influence miR 125b expression
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