MicroRNA-1-3p inhibits the growth and metastasis of ovarian cancer cells by targeting DYNLT3.

Abstract

For the purpose of exploring the specific mechanism of the oncogene dynein light chain Tctex-type 3 (DYNLT3) in OVC, bioinformatics techniques were applied to predict miRNAs that might bind to DYNLT3, and then microRNA-1-3p was selected. After measuring the expression levels of microRNA-1-3p and DYNLT3 in 60 pairs of OVC tissue samples, the Pearson correlation analysis was used to calculate the expression correlation of microRNA-1-3p and DYNLT3. In addition, Dual-Luciferase reporting assay was used to verify the combination of the two in OVC cells. Furthermore, microRNA-1-3p NC, microRNA-1-3p mimics, and microRNA-1-3p mimics+ DYNLT3-OE (overexpression) were transfected into ES-2 and SKOV-3 cells, respectively. Subsequently, real-time quantitative polymerase chain reaction (qPCR) was performed to examine microRNA-1-3p level in each group of cells, followed by cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) test, and transwell assay to verify the impact of microRNA-1-3p on the proliferation, migration ability, and invasiveness of OVC cells. Finally, the mRNA and protein levels of DYNLT3 were examined by qPCR and Western blot in OVC, respectively. Bioinformatics prediction results showed that a total of three possible miRNAs bound to the oncogene DYNLT3. Then, microRNA-1-3p was selected for further validation. qPCR results revealed that microRNA-1-3p was down-regulated in OVC tissues and cells, while DYNLT3 was up-regulated in OVC tissues. In addition, Pearson correlation analysis indicated that microRNA-1-3p was negatively correlated with DYNLT3 expression, and the Dual-Luciferase reporter assay confirmed that microRNA-1-3p was able to bind directly to the 3'-UTR of DYNLT3. Besides, microRNA-1-3p-mimics transfection remarkably decreased the mRNA and protein expressions of DYNLT3. On the contrary, transfection of microRNA-1-3p-mimics remarkably inhibited the proliferation, migration ability, and invasiveness of OVC cells. Moreover, the transfection of microRNA-1-3p-mimics+DYNLT3-OE partially reversed the inhibitory effect of microRNA-1-3p-mimics on the proliferative, migrate ability, and invasiveness of OVC cells. MicroRNA-1-3p is under expressed either in OVC tissues or in cell lines, and overexpression of microRNA-1-3p may inhibit proliferative and migrate ability and invasiveness of OVC cells by modulating DYNLT3, which make microRNA-1-3p a potential therapeutic target for OVC.