Abstract
Axillary buds of Maytenus aquifolium were cultured in Murashige and Skoog (MS) medium with growth regulators to induce plantlet formation. Shoot proliferation induced in MS medium supplemented 3 mg/l with 6-benzylaminopurine (BAP) was greater than in MS medium with kinetin at the same concentration. Plantlet height was also affected by both type and concentration of cytokinin. Addition of 0.2 mg/l of indole-3-acetic acid (IAA) to the medium contributed to an increase in plantlet height. Rootless plantlets were rooted successfully in vitro or ex vitro.
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