Abstract
The present study was carried out to study the micropropagation of Gardenia (Gardenia jasminoides) by using single nodes excised from soft cuttings using half strength MS salts, 30gl-1 sucrose, 7gl-1 Agar and different concentrations of plant growth regulators in culture medium. Results of the experiment at initiation stage revealed that the culture of single nodes of Gardenia on a medium containing 5mgl-1BA gave the highest number of shoots (2.2 shoots/explant). Concerning the interaction, a nutrient medium containing (2 mgl-1 BA+ 0.4 mgl-1 IAA) gave the highest values of average number of shoots and leaves and length of new shoots (1.6 shoots/explant, 2 leaves/explant, 1.6cm respectively). At vegetative multiplication stage, the results showed that the medium supplemented by 2 mgl-1 BA gave the highest values of average number of shoots and leaves and length of new shoots (2.20 shoots/explant, 3.20 leaves/explant, 1.80cm respectively), whereas the medium supplemented by 0.3 mgl-1 IAA gave the highest number of shoots and length of new shoots. In order to promote the shoots produced at vegetative multiplication stage to root, it was noticed that the treatment of 8mgl-1NAA gave the highest percentage of rooting (90%) and the medium supplemented by 12mgl-1 IAA gave (100%) rooting. Plantlets obtained were transferred to pots and acclimatized with 95 % success.
Highlights
Gardenia jasminoides or common Gardenia is a member of the family Rubiceae and belongs to the genus Gardenia
Two species are of primary importance, Gardenia jasminoides, containing many cultivars, and Gardenia thunbergia, grown primarily as a rootstock
Propagation could be done by grafting scion from a desired cultivar to a seedling rootstock of Gardenia thunbergia
Summary
Two species are of primary importance, Gardenia jasminoides, containing many cultivars, and Gardenia thunbergia, grown primarily as a rootstock. Gardenias can be used as screens, hedges, borders or ground covers. They may be used as free-standing specimens or in mass plantings. Cultivars of Gardenia jasminoides can be propagated by cuttings or grafting. Propagation could be done by grafting scion from a desired cultivar to a seedling rootstock of Gardenia thunbergia. Rootstock seedlings, they are difficult to obtain due to problems in seed germination. Pontikis and Sapoutzaki(1984) were able to get shoots grown on terminal buds of troyer citrange seedlings grown on MS medium enriched by 0.5 mgl-1BA, 0.1mgl-1 IBA and 0.1 mgl-1 GA3. Plant tissue culture is the growth of plant organs or tissues in aseptic culture where the environment as well as the nutrient and hormone levels for growth are tightly controlled (Razdan, 2003). Pontikis and Sapoutzaki(1984) were able to get shoots grown on terminal buds of troyer citrange seedlings grown on MS medium enriched by 0.5 mgl-1BA, 0.1mgl-1 IBA and 0.1 mgl-1 GA3. Pasqual and Audo (1989) promoted vegetative multiplication of tri-foliate orange shoots by using MS medium supplemented by 2 mgl-1 BA and 1mgl-1 NAA. Kyoichi et al (1987)
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