Abstract
Aloe polyphylla and A. calcairophila are two endangered species that are endemic to South Africa and Madagascar respectively. Generally ex situ conservation through in vitro culture involves growing plant material in nutrient rich media containing plant growth regulators to achieve shoot multiplication and rooting. In this study we report shoot multiplication and rooting of these two important plants in culture using half strength Murashige and Skoog (MS) medium containing glucose as the carbon source. Plants developed in glucose-containing medium were of superior quality in both species compared to those grown on standard carbon source, sucrose. This method has been applied to micro-propagate other species of Aloe at the Royal Botanic Gardens, Kew, England.
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