Microplastics and trace metals in fish species of the Gulf of Mannar (Indian Ocean) and evaluation of human health

Abstract

The importance of microplastic (MPs) contamination in marine environments is reflected by increasing number of studies in fish species. Some even dedicated to the toxicological effects from the ingestion. Microplastics (MPs) and their trace metal composition were examined in the muscle and intestine of five commercially important fish species (i.e., Sufflamen fraenatus, Heniochus acuminatus, Atropus atropos, Pseudotriacanthus and Leiognathus brevirostris) from Thoothukudi at the Gulf of Mannar coast in south India. The abundance and morphology of MPs (size, shape, and texture) in muscle and intestinal were investigated by micro-Fourier Transform Infrared Spectroscopy (μ-FT-IR) and atomic force microscope (AFM). ICP-OES was used to investigate the adsorption/leaching of trace metals in microplastics in order to assess health risk for adults and children. Particles of 100–250 μm and white color dominated, and the mean abundances (items/100 g) of total MPs were more in Pseudotriacanthus (muscle: 51.2; intestine: 50.1) compared to Heniochus acuminatus (muscle: 9.6; intestine: 15), Leiognathus brevirostris (muscle: 12; intestine: 13.2) and Atropus atropus (muscle: 15.2; intestine: 44.1). Polyethylene (35.3%), polypropylene (27.2%), polyamide (nylon) (22.2%) and fiber (15.3%) represented the MPs present in muscles, and polyamide (nylon) (30.2%), polyethylene (28.1%), polypropylene (25.9%), and fiber (15.8%) composed the intestine MPs. We estimated possible consumption of 121–456 items of MPs/week by adults and about 19–68 items of MPs/week by children by considering the sizes of safe meals. Zn, Cu, Mn and Cr in these fish species reflected influence of the sewage waste. However, the non-carcinogenic risk evaluated through EDI, THQ, HI, and CR did not suggest any immediate health problem for the consumers.